Table of Contents
ISRN Veterinary Science
Volume 2011 (2011), Article ID 548385, 8 pages
http://dx.doi.org/10.5402/2011/548385
Research Article

Single Layer Centrifugation with Androcoll-P Can Be Scaled-Up to Process Larger Volumes of Boar Semen

1Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences—SLU, P. O. Box 7054, 75007 Uppsala, Sweden
2Faculty of Veterinary Medicine, 3508TD Utrecht, The Netherlands
3Institute of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, P. O. Box 7011, 75007 Uppsala, Sweden
4Quality Genetics, Hörby, Sweden

Received 14 September 2010; Accepted 3 November 2010

Academic Editors: R. Gamble and Y. Yin

Copyright © 2011 Marjet van Wienen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objective of this study was to scale-up the procedure for Single Layer Centrifugation (SLC) through AndrocollTM-P, as a preliminary step towords processing the whole ejaculate. The first experiment compared Single Layer Centrifugation using 4.5 mL and 15 mL extended ejaculate (SLC-4.5 and SLC-15, resp.), assessing sperm quality by objective motility analysis, morphology, viability, and the production of reactive oxygen species (ROS). In the second experiment, SLC-4.5 was compared to Single Layer Centrifugation with 25 mL extended ejaculate (SLC-25) using motility analysis and morphology. In both experiments, normal morphology and linear motility were significantly higher in the SLC-selected samples than in the uncentrifuged controls ( 𝑃 < . 0 0 1 ), whereas total motility and membrane integrity were unchanged. Although ROS production was higher in the SLC-selected samples than in the controls ( 𝑃 < . 0 1 ), this might have been due to the presence of antioxidants in seminal plasma in the latter. In conclusion, there was no difference in sperm quality between SLC-4.5 and SLC-15 samples, or between SLC-4.5 and SLC-25 samples, indicating that the SLC method can be scaled-up successfully.