Review Article

The Central Role of Glia in Pathological Pain and the Potential of Targeting the Cannabinoid 2 Receptor for Pain Relief

Figure 1

Qualitative confocal images of cellular immunostaining. (a) Immunostaining of Iba-1 (red) for infiltrating macrophages and microglia in the meninges and superficial white matter of the spinal cord in a rat with ongoing neuropathy. (b) Immunostaining with IL-1β (green). (c) Arrows indicate yellow colabeling of IL-1β- and Iba-1-positive cells and not with GFAP (blue). (d) Immunostaining of Iba-1 (red) in the deeper laminae of the spinal cord dorsal horn in a rat with ongoing neuropathy. (e) Immunostaining with IL-1β (green). (f) Arrows indicate yellow colabeling of IL-1β- and Iba-1-positive cells and not with GFAP (blue). (g) DRG immunostaining of GFAP positive satellite cells (red) and neurons stained for neurofilament-heavy (NF-H, white) from a rat with ongoing neuropathy. (h) DRG immunostaining for IL-1β (green). (i) Arrows indicate yellow DRG IL-1β and GFAP colabeling with DAPI nuclear labeling (blue). (j) Immunostaining of Iba-1 (red) in meninges and superficial laminae of the dorsal horn spinal cord in a rat with ongoing neuropathy. (k) Immunostaining of MAGL (green). (l) Arrows indicate yellow colabeling of MAGL and Iba-1-positive cells, and not with GFAP (blue). Scale bars for all images indicate 20 μm.
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