Table of Contents
ISRN Urology
Volume 2011, Article ID 605436, 5 pages
http://dx.doi.org/10.5402/2011/605436
Research Article

Iron Inhibits Respiratory Burst of Peritoneal Phagocytes In Vitro

1Department of General Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Cracow, Poland
2Molecular Neuropharmacology Group, Department of Neuroscience and Pharmacology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Building 18.6, 2200 Copenhagen N, Denmark
3Department of Nephrology and Dialysis, Rydygier Hospital, Złota Jesień 1, 31-826 Cracow, Poland

Received 15 August 2011; Accepted 15 September 2011

Academic Editors: J. R. Drevet, A. M. El-Assmy, and F. M. Wagenlehner

Copyright © 2011 Kamil Gotfryd et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. This study examines the effects of iron ions Fe3+ on the respiratory burst of phagocytes isolated from peritoneal effluents of continuous ambulatory peritoneal dialysis (CAPD) patients, as an in vitro model of iron overload in end-stage renal disease (ESRD). Material and Methods. Respiratory burst of peritoneal phagocytes was measured by chemiluminescence method. Results. At the highest used concentration of iron ions Fe3+ (100 μM), free radicals production by peritoneal phagocytes was reduced by 90% compared to control. Conclusions. Iron overload may increase the risk of infectious complications in ESRD patients.