Table of Contents
ISRN Obstetrics and Gynecology
Volume 2011, Article ID 692858, 5 pages
Research Article

Regulatory Effect of Peptidoglycan on the Expression of Toll-Like Receptor 2 mRNA and Proteins in Trophoblast Cell Line TEV-1 Cells

1Department of Gynaecology, Guangdong Women and Children's Hospital and Health Institute, Guangzhou, 510010, China
2South Western Sydney Clinical School, Faculty of Medicine, University of New South Wales, P.O. Box 3256, Blakehurst, Sydney NSW 2221, Australia
3Faculty of Medicine, University of New South Wales, Sydney NSW 2052, Australia
4Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430074, China

Received 28 August 2010; Accepted 21 September 2010

Academic Editors: E. G. Olivares and K. Yang

Copyright © 2011 Yi Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. To investigate the regulatory effect of peptidoglycan on the expression of human Toll-like receptors 2 (TLR2) mRNA and proteins in the human extravillous trophoblast cell line (TEV-1). Methods. TEV-1 cells were incubated with different doses of peptidoglycan. The expression of TLR2 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunocytochemistry SP staining. Results. TLR2 was expressed in TEV-1 cells and localized to both the cytoplasm and plasma membrane. Compared with the untreated control, TEV-1 cells incubated with 30  πœ‡ g/ml peptidoglycan significantly upregulated the expression of TLR2 mRNA and protein after 12 hours of treatment ( 𝑃 < . 0 1 ). However, the expression of TLR2 mRNA and protein was decreased but had no significant difference compared with the control ( 𝑃 > . 0 5 ) after 24 hours of treatment. On the other hand, 10  πœ‡ g/ml peptidoglycan did not seem to have regulatory effect on mRNA and protein expression of TLR2 ( 𝑃 > . 0 5 ). Conclusion. Peptidoglycan has a role in regulating the expression of TLR2 mRNA and protein in TEV-1 cells. It suggests that the trophoblast cells may play important role in the immune response at the fetal-maternal interface and affect the result of pregnancy by expressing TLR2.