Structural and Functional Characterization of a New Double Variant Haemoglobin (HbG-Philadelphia/Duarte 𝛼^{𝟔𝟖Asn→Lys}_𝟐𝛽^{𝟔𝟐Ala→Pro}_𝟐)

Figure 3

Identification of variant hemoglobins. (a) IEF of hemolysate variant (lane 1), HbA (lane 2), purified Hb Duarte (lane 3), and purified Hb G-Philadelphia/Duarte (lane 4); RP-HPLC of the globin chains of (b) hemolysate variant; (c) purified Hb Duarte; (d) purified Hb G-Philadelphia/Duarte.