Table of Contents
ISRN Cardiology
Volume 2011, Article ID 740585, 5 pages
Research Article

Insulin Receptor Substrate-1 Expression Is Increased in Circulating Leukocytes of Patients with Acute Coronary Syndrome

1Department of Cardiology, Hospital Clínico Universitario Virgen de la Victoria, 29010 Málaga, Spain
2Department of Cardiology, Hospital General Universitario Gregorio Marañón, 28007 Madrid, Spain
3Department of Internal Medicine, Hospital General Universitario Gregorio Marañón, 28007 Madrid, Spain
4Department of Epidemiology, Atherothrombosis and Imaging, Spanish National Cardiovascular Research Center (CNIC), 28029 Madrid, Spain
5Instituto de Biomedicina de Valencia (IBV), Spanish Council for Scientific Research (CSIC) and Fundación Investigación Hospital Clínico de Valencia, Av. Blasco Ibáñez, 46010 Valencia, Spain

Received 21 February 2011; Accepted 20 April 2011

Academic Editor: K. Nakamura

Copyright ยฉ 2011 Manuel F. Jimรฉnez-Navarro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The mechanisms underlying the increased risk of cardiovascular disease associated with diabetes mellitus (DM) are not fully defined. Insulin resistance in human metabolic syndrome patients is associated with decreased expression of the insulin receptor substrate-2- (Irs2-) AKT2 axis in mononuclear leukocytes (MLs). Moreover, acute coronary syndrome (ACS) has been linked through genome-wide association studies to the 2q36-q37.3 locus, which contains the Irs1 gene. Here, we investigated the expression of insulin-signaling pathway genes in MLs from patients with DM, ACS, and ACS plus DM. Quantitative real-time PCR expression studies showed no differences in the mRNA levels of Irs2, Akt2, and Akt1 among all patients. However, Irs1 mRNA expression was significantly increased in patients with ACS—diabetics and nondiabetics—compared with diabetic patients without ACS ( ๐‘ƒ < . 0 2 and ๐‘ƒ < . 0 0 5 , resp.). The present study reveals for the first time an association between increased Irs1 mRNA levels in MLs of patients with ACS which is not related to DM.