Table of Contents
ISRN Pharmaceutics
Volume 2011 (2011), Article ID 780150, 7 pages
http://dx.doi.org/10.5402/2011/780150
Research Article

Development and Evaluation of Microemulsions for Transdermal Delivery of Insulin

1Department of Pharmaceutics, Bengal College of Pharmaceutical Sciences and Research, West Bengal, Durgapur 713212, India
2Department of Pharmaceutics, Gupta College of Technological Sciences, West Bengal, Asansol 713301, India
3Department of Pharmaceutics, Seemanta Institute of Pharmaceutical Sciences, Orissa, Mayurbhanj 757086, India

Received 5 April 2011; Accepted 16 May 2011

Academic Editors: C. V. Garcia, K. Pudhom, and C. Ferrero Rodríguez

Copyright © 2011 Jadupati Malakar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Insulin-loaded microemulsions for transdermal delivery were developed using isopropyl myristate or oleic acid as the oil phase, Tween 80 as the surfactant, and isopropyl alcohol as the cosurfactant. The pseudoternary phase diagrams were constructed to determine the composition of microemulsions. The insulin permeation flux of microemulsions containing oleic acid as oil phase through excised mouse skin and goat skin was comparatively greater than that of microemulsions containing isopropyl myristate as oil phase. The insulin-loaded microemulsion containing 10% oleic acid, 38% aqueous phase, and 50% surfactant phase with 2% dimethyl sulfoxide (DMSO) as permeation enhancer showed maximum permeation flux (4.93 ± 0.12 μg/cm2/hour) through goat skin. The in vitro insulin permeation from these microemulsions was found to follow the Korsmeyer-Peppas model ( 𝑅 2 = 0 . 9 2 3 to 0.973) over a period of 24 hours with non-Fickian, “anomalous” mechanism. Together these preliminary data indicate the promise of microemulsions for transdermal delivery of insulin.