Proteases in Plasma and Kidney of db/db Mice as Markers of Diabetes-Induced Nephropathy
Gelatin zymography and Western blotting of kidney extracts from db/db and db/+ mice. (a) kidney extracts from three different db/+ mice (Control) and db/db mice (Diabetes) were subjected to zymography. The molecular size standards used are the same as those described in the legend to Figure 1. (b) extracts from db/+ (C) and db/db (D) mice were also subjected to gelatin zymography with or without EDTA (10 mM) or Pefabloc (1 mmol/L) in the washing and incubation buffers. Molecular size standards used were as described in the legend to Figure 1. TR: Trypsin. (c) extracts from db/+ (Control) and db/db (Diabetes) mice were subjected to lentil lectin-Sepharose chromatography followed by Western blotting using an antibody against MMP-9. The arrow shows the migration of the pro-MMP-9 (92000) standard.