Expression of the Lactate Dehydrogenase Gene from Eptatretus okinoseanus in Escherichia coli

Figure 1

Genetic engineering scheme for construction of E. okinoseanus LDH-A open reading frame (ORF) and pCold TF vector. The E. okinoseanus LDH-A ORF was inserted asymmetrically into the NdeI and EcoRI sites of the pCold TF vector into which a csp A promoter. The pCold TF vector with LDH includes a translation enhancing element (TEE), a His-tag sequence, and three sites cleaved by proteases (HRV 3C protease, Thrombin, and Factor Xa).