Table of Contents
ISRN Dermatology
Volume 2011 (2011), Article ID 848909, 8 pages
http://dx.doi.org/10.5402/2011/848909
Research Article

Immunohistochemical Reactivity of the 14F7 Monoclonal Antibody Raised against N-Glycolyl GM3 Ganglioside in Some Benign and Malignant Skin Neoplasms

1Department of Quality Control, Center of Molecular Immunology, Havana 11600, Cuba
2Department of Pathology, Manuel Fajardo General Hospital, Havana 10400, Cuba
3Research and Development Direction, Center of Molecular Immunology, 216 Street, 15 Avenue, Atabey, Playa, P.O. Box 16040, Havana 11600, Cuba

Received 11 February 2011; Accepted 22 March 2011

Academic Editors: F. Fabrizio and D. Watanabe

Copyright © 2011 Rancés Blanco et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The evaluation of 14F7 Mab (anti-N-glycolyl GM3 ganglioside) immunorecognition in normal skin, cutaneous malignant melanoma (CMM), and in lymph node metastases (LNM) has been previously reported. In this work we extended the study to benign (BMN) and dysplastic (DMN) melanocytic nevi, basal (BCC), and squamous cell carcinoma (SCC). Immunohistochemical assays with 14F7 followed by a biotinylated link universal and streptavidin-AP in normal and pathological tissues were made. No reaction of 14F7 in normal skin (0/10) as well as a low reactivity in BMN (2/11) and DMN (1/7) was detected. A limited staining in BCC (2/13) and in SCC (4/8) was also evidenced, while 14F7 Mab were mostly reactive in CMM (28/28) and in LNM (6/7). These results suggest that 14F7 reactivity could be closely related with the more aggressive biological behavior of CMM and also support the use of NeuGcGM3 as target for both passive and active melanoma immunotherapy.