Table of Contents
ISRN Pharmaceutics
Volume 2011, Article ID 860109, 6 pages
Research Article

Ex Vivo Evaluation of Insulin Nanoparticles Using Chitosan and Arabic Gum

1Faculty of Pharmacy, Azad University of Medical Sciences, Tehran, Iran
2Hakim Pharmaceutical Company, P.O. Box 11365-5465, Tehran, Iran
3Razi Vaccine and Serum Research Institute, P.O. Box 3197619751 Karaj, Iran
4Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Received 4 April 2011; Accepted 5 May 2011

Academic Editors: A. A. Abdel-Aziz, Y. Murata, and A. S. Zidan

Copyright © 2011 M. R. Avadi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Polymeric delivery systems based on nanoparticles have emerged as a promising approach for peroral insulin delivery. The aim of the present study was to investigate the release of insulin nanoparticulate systems and ex vivo studies. The nanoparticles were prepared by the ion gelation method. Particle size distribution, zeta potential, and polydispersity index of the nanoparticles were determined. It was found that the nanoparticles carried positive charges and showed a size distribution in the range of 170–200 nm. The electrostatic interactions between the positively charged group of chitosan and negatively charged groups of Arabic gum play an important role in the association efficiency of insulin in nanoparticles. In vitro insulin release studies showed an initial burst followed by a slow release of insulin. The mucoadhesion of the nanosystem was evaluated using excised rat jejunum. Ex vivo studies have shown a significant increase in absorption of insulin in the presence of chitosan nanoparticles in comparison with free insulin.