Table of Contents
ISRN Microbiology
Volume 2011, Article ID 938347, 5 pages
Research Article

Improved Mannanase Production from Penicillium occitanis by Fed-Batch Fermentation Using Acacia Seeds

1Unité Enzymes et Bioconversion, Ecole National d'ingénieurs de Sfax, Université de Sfax, Route de Soukra 3038, Sfax, Tunisia
2Unité de Service Commun Bioréacteur Couplé à un Ultrafiltre, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, Route de Soukra 3038, Sfax, Tunisia

Received 10 July 2011; Accepted 8 August 2011

Academic Editors: S. H. Flint, T. B. Karegoudar, G. Suzzi, and T. P. West

Copyright © 2011 Monia Blibech et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase production using acacia seeds powder as inducer substrate showed the suitability of this culture process for industrial-scale development.