Table of Contents
ISRN Pharmaceutics
Volume 2011 (2011), Article ID 948314, 4 pages
http://dx.doi.org/10.5402/2011/948314
Research Article

Development and Validation of a HPLC and an UV Spectrophotometric Methods for Determination of Dexibuprofen in Pharmaceutical Preparations

Department of Pharmaceutical Analysis, JSS College of Pharmacy, Ootacamund, Tamilnadu-643 001, India

Received 12 April 2011; Accepted 16 May 2011

Academic Editors: G. N. C. Chiu and F. de la Calle

Copyright © 2011 Selvadurai Muralidharan and Subramania Nainar Meyyanathan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A high-performance liquid chromatographic (HPLC) and a ultraviolet (UV) methods were developed and validated for the quantitative determination of Dexibuprofen (DI) in pharmaceutical dosage form. HPLC was carried out by reversed phase technique on a RP-18 column with a mobile phase composed of acetonitrile and 0.5% triethylamine (pH 7.5 adjusted with orthophosphoric acid (30 : 70, v/v)). UV method was performed with the λ max at 222.0 nm. Both the methods showed good linearity, reproducibility and precision. No spectral or chromatographic interferences from the tablet excipients were found in UV and HPLC. The method was successfully applied to commercial DEXIFEN tablets. Validation parameters such as linearity, precision, accuracy, and specificity were determined. The proposed method could be applicable for routine analysis of DI and monitoring of the quality of marketed drugs.