Table of Contents
ISRN Urology
Volume 2012 (2012), Article ID 108576, 6 pages
http://dx.doi.org/10.5402/2012/108576
Clinical Study

Increased Fertilization Rates after In Vitro Culture of Frozen-Thawed Testicular Immotile Sperm in Nonobstructive Azoospermic Patients

Reproduction Unit, CLTNTCA Tambre, Calle Tambre no. 8, 28002 Madrid, Spain

Received 30 November 2011; Accepted 19 December 2011

Academic Editors: B. Altay and G. Haidl

Copyright © 2012 R. Nuñez-Calonge et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. Schoysman, P. Vanderzwalmen, M. Nijs et al., “Pregnancy after fertilisation with human testicular spermatozoa,” Lancet, vol. 342, no. 8881, p. 1237, 1993. View at Google Scholar · View at Scopus
  2. A. Van Steirteghem, Z. Nagy, J. Liu et al., “Intracytoplasmic single sperm injection with testicular sperm cell,” Annalen, vol. 2, pp. 8–10, 1993. View at Google Scholar
  3. P. Devroey, J. Liu, Z. Nagy et al., “Normal fertilization of human oocytes after testicular sperm extraction and intracytoplasmic sperm injection,” Fertility and Sterility, vol. 62, no. 3, pp. 639–641, 1994. View at Google Scholar · View at Scopus
  4. S. J. Silber, A. C. Van Steirteghem, Z. Nagy, J. Liu, H. Tournaye, and P. Devroey, “High fertilization and pregnancy rate after intracytoplasmic sperm injection with spermatozoa obtained from testicle biopsy,” Human Reproduction, vol. 10, no. 1, pp. 148–152, 1995. View at Google Scholar · View at Scopus
  5. L. Gianaroli, M. C. Magli, H. A. Selman et al., “Diagnostic testicular biopsy and cryopreservation of testicular tissue as an alternative to repeated surgical openings in the treatment of azoospermic men,” Human Reproduction, vol. 14, no. 4, pp. 1034–1038, 1999. View at Google Scholar · View at Scopus
  6. G. S. Prins, R. Dolgina, P. Studney, B. Kaplan, L. Ross, and C. Niederberger, “Quality of cryopreserved testicular sperm in patients with obstructive and nonobstructive azoospermia,” Journal of Urology, vol. 161, no. 5, pp. 1504–1508, 1999. View at Publisher · View at Google Scholar · View at Scopus
  7. J. Liu, Y. L. Tsai, E. Katz, G. Compton, J. E. Garcia, and T. A. Baramki, “Outcome of in-vitro culture of fresh and frozen-thawed human testicular spermatozoa,” Human Reproduction, vol. 12, no. 8, pp. 1667–1672, 1997. View at Publisher · View at Google Scholar · View at Scopus
  8. H. Habermann, R. Seo, J. Cieslak, C. Niederberger, G. S. Prins, and L. Ross, “In vitro fertilization outcomes after intracytoplasmic sperm injection with fresh or frozen-thawed testicular spermatozoa,” Fertility and Sterility, vol. 73, no. 5, pp. 955–960, 2000. View at Publisher · View at Google Scholar · View at Scopus
  9. B. Balaban, B. Urman, A. Sertac et al., “In-vitro culture of spermatozoa induces motility and increases implantation and pregnancy rates after testicular sperm extraction and intracytoplasmic sperm injection,” Human Reproduction, vol. 14, no. 11, pp. 2808–2811, 1999. View at Publisher · View at Google Scholar · View at Scopus
  10. S. Emiliani, M. D. Van Bergh, A. S. Vannin, J. Biramane, M. Verdoodt, and Y. Englert, “Increased sperm motility after in-vitro culture of testicular biopsies from obstructive azoospermic patients results in better post-thaw recovery rate,” Human Reproduction, vol. 15, no. 11, pp. 2371–2374, 2000. View at Google Scholar · View at Scopus
  11. M. Nijs, B. Segal-Bertin, P. Vandamme et al., “In vitro culture of testicular spermatozoa: an improvement for the ICSI outcome?” Journal of Assisted Reproduction and Genetics, vol. 14, supplement, p. 75S, 1997. View at Google Scholar
  12. J. Konc, K. Kanyó, and S. Cseh, “The effect of condition/state of testicular spermatozoa injected to the outcome of TESE-ICSI-ET cycles,” European Journal of Obstetrics & Gynecology and Reproductive Biology, vol. 141, no. 1, pp. 39–43, 2008. View at Google Scholar
  13. P. Devroey, P. Nagy, H. Tournaye, J. Liu, S. Silber, and A. Van Steirteghem, “Outcome of intracytoplasmic sperm injection with testicular spermatozoa in obstructive and non-obstructive azoospermia,” Human Reproduction, vol. 11, no. 5, pp. 1015–1018, 1996. View at Google Scholar · View at Scopus
  14. J. Liu, J. E. Garcia, G. Compton et al., “Pregnancy after use of frozen-thawed human testicular spermatozoa by using intracytoplasmic sperm injection,” in Proceedings of the 52nd Annual Meeting of American Society for Reproductive Medicine, p. S95, Boston, Mass, USA, November 1996.
  15. H. Nagase and T. Niwa, “Deep freezing of bull semen in concentrated pellet form. I. Factors affecting survival of spermatozoa,” in Proceedings of the 5th International Congress on Animal Reproduction, vol. 4, Trento, Italy, 1964.
  16. P. Caballero, R. Núñez, and I. Vázquez, “Coordinación de un Banco de Semen,” in Fertilidad, Análogos Gn RH-Periconceptología-Endoscopia, J. M. Dexeus and P. N. Barri, Eds., pp. 83–92, Masson-Salvat Medicina, 1993. View at Google Scholar
  17. R. Nunez-Calonge, S. Cortes, M. Gago, A. García Segovia, and P. Peramo and Caballero, “Optimización de los resultados de microinyección intracitoplasmática con espermatozoides congelados y descongelados procedentes de biopsia de testículo,” Revista Internacional de Andrología, vol. 5, no. 4, pp. 343–348, 2007. View at Google Scholar
  18. M. Gil-Salom, J. Romero, Y. Mínguez et al., “Pregnancies after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa,” Human Reproduction, vol. 11, no. 6, pp. 1309–1313, 1996. View at Google Scholar · View at Scopus
  19. W. Küpker, P. N. Schlegel, S. Al-Hasani et al., “Use of frozen-thawed testicular sperm for intracytoplasmic sperm injection,” Fertility and Sterility, vol. 73, no. 3, pp. 453–458, 2000. View at Publisher · View at Google Scholar · View at Scopus
  20. M. H. Lin, M. Morshedi, C. Srisombut, A. Nassar, and S. Oehninger, “Plasma membrane integrity of cryopreserved human sperm: an investigation of the results of the hypoosmotic swelling test, the water test, and eosin-Y staining,” Fertility and Sterility, vol. 70, no. 6, pp. 1148–1155, 1998. View at Publisher · View at Google Scholar · View at Scopus
  21. J. A. Allan and A. S. Cotman, “A new method for freezing testicular biopsy sperm: three pregnancies with sperm extracted from cryopreserved sections of seminiferous tubule,” Fertility and Sterility, vol. 68, no. 4, pp. 741–744, 1997. View at Publisher · View at Google Scholar · View at Scopus
  22. E. Crabbé, G. Verheyen, H. Tournaye, and A. Van Steirteghem, “Freezing of testicular tissue as a minced suspension preserves sperm quality better than whole-biopsy freezing when glycerol is used as cryoprotectant,” International Journal of Andrology, vol. 22, no. 1, pp. 43–48, 1999. View at Publisher · View at Google Scholar · View at Scopus
  23. J. Zhu, G. Meniru, and I. Craft, “In vitro maduration of human testicular spermatozoa in patients with azoospermia,” Journal of Assisted Reproduction and Genetics, vol. 14, pp. 361–363, 1997. View at Google Scholar
  24. P. Terriou, E. Hans, C. Giorgetti et al., “Pentoxifylline initiates motility in spontaneously immotile epididymal and testicular spermatozoa and allows normal fertilization, pregnancy, and birth after intracytoplasmic sperm injection,” Journal of Assisted Reproduction and Genetics, vol. 17, no. 4, pp. 194–199, 2000. View at Publisher · View at Google Scholar · View at Scopus
  25. T. M. Aktan, M. Montag, S. Duman, H. Gorkemli, K. Rink, and T. Yurdakul, “Use of a laser to detect viable but immotile spermatozoa,” Andrologia, vol. 36, no. 6, pp. 366–369, 2004. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  26. N. M. de Oliviera, V. Sanchez, S. R. Iesta et al., “Pregnancy with frozen-thawed and fresh tes- ticular biopsy after motile and immotile sperm microinjection, using the mechanical touch technique to assess viability,” Human Reproduction, vol. 19, pp. 262–265, 2004. View at Google Scholar
  27. R. F. Casper, J. S. Meriano, K. A. Jarvi, L. Cowan, and M. L. Lucato, “The hypo-osmotic swelling test for selection of viable sperm for intracytoplasmic sperm injection in men with complete asthenozoospermia,” Fertility and Sterility, vol. 65, no. 5, pp. 972–976, 1996. View at Google Scholar · View at Scopus
  28. H. Tournaye, M. Van der Linden, E. Van den Abbeel, P. Devroey, and A. Van Steirteghem, “Effects of pentoxifylline on in-vitro development of preimplantation mouse embryos,” Human Reproduction, vol. 8, no. 9, pp. 1475–1480, 1993. View at Google Scholar · View at Scopus
  29. S. M. Downs and J. J. Eppig, “The role of purines in the maintenance of meiotic arrest in mouse oocytes,” Tokai Journal of Experimental and Clinical Medicine, vol. 11, no. 6, pp. 463–469, 1986. View at Google Scholar · View at Scopus
  30. H. Tournaye, M. Van der Linden, E. Van den Abbeel, P. Devroey, and A. Van Steirteghem, “Effect of pentoxifylline on implantation and post-implantation development of mouse embryos in vitro,” Human Reproduction, vol. 8, no. 11, pp. 1948–1954, 1993. View at Google Scholar · View at Scopus
  31. H. Tournaye, A. C. Van Steirteghem, and P. Devroey, “Pentoxifylline in idiopathic male-factor infertility: a review of its therapeutic efficacy after oral administration,” Human Reproduction, vol. 9, no. 6, pp. 996–1000, 1994. View at Google Scholar · View at Scopus
  32. D. Loutradis, D. John, and A. A. Kiessling, “Hypoxanthine causes a 2-cell block in random-bred mouse embryos,” Biology of Reproduction, vol. 37, no. 2, pp. 311–316, 1987. View at Google Scholar · View at Scopus
  33. S. M. Downs, “Purine control of mouse oocyte maturation: evidence that nonmetabolized hypoxanthine maintains meiotic arrest,” Molecular Reproduction and Development, vol. 35, no. 1, pp. 82–94, 1993. View at Google Scholar · View at Scopus
  34. L. Scott and S. Smith, “Human sperm motility-enhancing agents have detrimental effects on mouse oocytes and embryos,” Fertility and Sterility, vol. 63, no. 1, pp. 166–175, 1995. View at Google Scholar · View at Scopus
  35. T. Angelopoulos, A. Adler, L. Krey, F. Licciardi, N. Noyes, and A. McCullough, “Enhancement or initiation of testicular sperm motility by in vitro culture of testicular tissue,” Fertility and Sterility, vol. 71, no. 2, pp. 240–243, 1999. View at Publisher · View at Google Scholar · View at Scopus
  36. G. Verheyen, Z. Nagy, H. Joris, I. De Croo, H. Tournaye, and A. Van Steirteghem, “Quality of frozen-thawed testicular sperm and its preclinical use for intracytoplasmic sperm injection into in vitro-matured germinal-vesicle stage oocytes,” Fertility and Sterility, vol. 67, no. 1, pp. 74–80, 1997. View at Publisher · View at Google Scholar · View at Scopus
  37. S. H. Maier, T. Nesslauer, K. Sterzik, and R. E. Hautmann, “Fresh sperms are superior for assisted fertilization (ICSI),” Journal of Urology, vol. 161,abstrac no. 1339, pp. 346–348, 1999. View at Google Scholar