Prediction of Total Phenolic Content in Extracts of Prunella Species from HPLC Profiles by Multivariate Calibration

Table 1

Total phenol contents of Prunella L. species using different solvents.

Extraction solvent	Prunella vulgaris L. (mg GAE g^−1 dried plant)	Prunella laciniata (L.) L. (mg GAE g^−1 dried plant)	Prunella orientalis Bornm. (mg GAE g^−1 dried plant)	Prunella grandiflora L. (mg GAE g^−1 dried plant)
Water	15.48±0.03	20.21±1.35	11.00±0.12	24.63±0.55
Methanol	11.52±0.55	11.69±1.36	8.44±0.56	16.93±0.02
Butanol	13.97±0.70	13.02±0.92	9.65±0.64	12.56±0.46
Acetonitrile	4.18±0.28	1.97±0.07	1.66±0.05	1.82±0.03
Ethyl acetate	2.09±0.28	1.76±0.02	1.89±0.63	2.84±0.07
Hexane	1.00±0.26	1.00±0.17	1.00±0.19	1.01±0.07
Acidic water	26.49±0.60	32.34±1.18	27.09±0.88	30.92±0.42
Acidic methanol	76.30±0.96	77.02±0.54	62.96±0.77	78.00±0.46
Acidic butanol	47.09±0.73	55.81±0.93	45.41±1.19	67.99±0.65
Acidic acetonitrile	76.51±0.91	77.33±0.76	63.52±0.63	79.65±0.70
Acidic ethyl acetate	87.33±0.91	86.07±0.86	70.56±0.57	83.95±0.39
Acidic hexane	1.24±0.07	1.26±0.09	1.59±0.08	1.41±0.37

Mean of two determinations ± SD (standard deviation).