Reprogramming of Human Huntington Fibroblasts Using mRNA

Figure 2

iPS pluripotency of cells derived from adult fibroblasts. (a) Morphology of mRNA-induced iPS cells (passages 0 and 1) and an ALP-stained mRNA-iPS colony. (b) Human fibroblasts from two different donors (Huntington patients) were transfected with different factor combinations including oct4 + sox2 + klf4 (OSK), oct4 + nanog + sox2 (ONS), oct4 + nanog + hTERT (ONT), oct4 + nanog + c-myc (ONM), oct4 + nanog + klf4 (ONK), and oct4 + nanog + sox2 + hTERT + klf4 + c-myc (ONSTKM). Colonies were counted 1 week after derivation (~day 30, cf. Figure 1). (c) Immunofluorescence staining for Oct4, Sox2, Nanog, SSEA4, SSEA3, and Tra-1-60 in mRNA-iPS cells derived from human (female, 21 years) fibroblasts.

(a) Morphology—adult mRNA-iPS

(b) Colony efficiency—Huntington mRNA-iPS

(c) Adult mRNA-iPS cells—Pluripotency