Research Article

An Assessment of Epithelial and Mesenchymal Phenotypes in Experimental and Clinical Pulmonary Fibrosis

Figure 4

Evidence of increased collagen deposition and changes in epithelial, mesenchymal, and apoptotic gene expression in bleomycin-treated mice. Mice were given bleomycin sulphate (0.015โ€‰U/30โ€‰ ๐œ‡ L/mouse) or saline i.t. at day 0 and then at days 3, 7, and 14, lungs were removed for subsequent analysis. Lung collagen was measured using hydroxyproline assay in homogenates from whole lung (a). RNA was isolated from whole lung and qRT-PCR was performed to identify EMT (b) and proapoptotic (c) regulated genes. Data points represent mean ยฑ SEM of at least ๐‘› = 3 mice at each time point. * ๐‘ƒ < 0 . 0 5 , *** ๐‘ƒ < 0 . 0 0 5 using Studentโ€™s t-test.