162802.fig.004a
(a)
162802.fig.004b
(b)
162802.fig.004c
(c)
Figure 4: Mitochondrial complex activity, as measured by spectrophotometry. (a) There is a significant decrease in complex I activity in high-glucose exposed renal MECs compared to low-glucose and high-glucose + C-peptide at both 24- and 48-hours. *, significantly less than low-glucose and high-glucose + C-peptide ( 𝑃 < 0 . 0 0 2 at 24 hr and 𝑃 < 0 . 0 0 0 9 at 48 hr). #, significantly greater than high glucose at 48 hr ( 𝑃 < 0 . 0 5 ). (b) and (c) Both complex III and IV activities are not different among the treatment conditions. Data represent mean ± SEM; 𝑛 = 1 6 experiments.