Table of Contents
ISRN Microbiology
Volume 2012, Article ID 162816, 4 pages
http://dx.doi.org/10.5402/2012/162816
Research Article

Comparison of the Serological Reactivity of Lipopolysaccharides from Japanese and Western Strains of Helicobacter pylori to Sera from H. pylori-Positive Humans

1Bioscience Education and Research Center, Akita University, 1-1-1, Hondo, Akita 010-8543, Japan
2Department of Microbiology, Sapporo Medical University School of Medicine, South-1, West-17, Chuo-ku, Sapporo 060-8556, Japan
3Department of Chemistry, University of Guelph, Guelph, ON, Canada N1G 2W1

Received 28 April 2012; Accepted 11 June 2012

Academic Editors: H. I. Atabay, C. K. Cote, and D. Rodriguez-Lazaro

Copyright © 2012 Ken-ichi Amano et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We compared the serological reactivity of lipopolysaccharides (LPS) isolated from Japanese and Western strains of Helicobacter pylori against anti-Lewis antigen monoclonal antibodies and H. pylori-positive Japanese sera. The two LPS from Western strains (26695 and O:2) did not react with any sera from Japanese patients, while all LPS from Japanese strains and the Sydney strain reacted with these sera. We propose that LPS of all Japanese smooth strains share either one of two epitopes, which are termed highly antigenic and weakly antigenic epitopes, present in the O-polysaccharide portion, and these epitopes are independent the Lewis antigens. The present findings indicated that the two Western strains lacked the two epitopes, which are shared by all Japanese strains.