Lipid Droplet Binding of Hepatitis C Virus Core Protein Genotype 3
Figure 1
Overview of GFP fusion constructs. (a) GFP fusion constructs were generated with various full-length and deletion constructs of HCV Core protein. Each construct is presented with the amino acid residue boundaries included. GFP-FL Core: full-length Core protein, GFP-d1-2: Core domains 1 and 2, GFP-d2-3: Core domains 2 and 3, GFP-d2: Core domain 2 alone, and GFP-d3: Core domain 3 alone. (Construct labels used in the figure are consistent through all figures.) (b) Construct expression was validated using transient transfection of Huh7.5 cells and western blotting. Each construct produced a band at the predicted protein size.
