Table of Contents
ISRN Microbiology
Volume 2012, Article ID 186915, 5 pages
http://dx.doi.org/10.5402/2012/186915
Research Article

Detection and Typing of Human Papilloma Virus by Multiplex PCR with Type-Specific Primers

FRP Genética, 115A Ote 1417-1, 72520 Puebla, PUE, Mexico

Received 12 October 2011; Accepted 23 November 2011

Academic Editors: T. Krishnan, A. Mirazimi, and N. Yamamoto

Copyright © 2012 Francisco Romero-Pastrana. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The primary underlying cause of cervical cancer is infection with one or more high-risk (HR) types of the human papilloma virus (HPV). Detection and typing of HPV have been commonly carried out by PCR-based assays, where HPV detection and typing are two separate procedures. Here, we present a multiplex PCR-based HPV typing assay that detects 20 HPV types (15 HR, 3 probably HR and 2 low risk) using type-specific primers and agarose gel electrophoresis. 46 cervical, urethral, and biopsy samples were analyzed by both Multiplex PCR and PGMY09/11 consensus PCR, and results were compared. 611 samples were further analyzed by Multiplex PCR, 282 were positive for HR HPV, and 101 showed multiple HR HPV infections. The relatively ease and economic accessibility of the method and its improved ability to detect high-risk HPV types in multiple HPV-infected samples make it an attractive option for HPV testing.