CCN1 Induces 𝜷-Catenin Translocation in Esophageal Squamous Cell Carcinoma through Integrin 𝜶11

Figure 2

Knockdown of CCN1 in OE21 ESCC cells (OE2^CCN1−) reduces the level of 𝛽-catenin. (a) Under a dual filter, the shRNA transfected cells display bright red fluorescence due to expression of RFP, while 𝛽-catenin is identified in green by FITC-conjugated antibody. (b) In the same view under FITC filter, the shRNA transfected cells display low level of 𝛽-catenin compared to nontransfected cells. (c) Western blot analysis shows reduced expression of 𝛽-catenin in OE21^CCN1− cells.

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