Octanoylated Ghrelin Inhibits the Activation of the Palmitic Acid-Induced TLR4/NF-B Signaling Pathway in THP-1 Macrophages
Figure 5
Acylated ghrelin pretreatment decreased palmitic acid-induced level of TLR4 protein and NF-B p65 phosphorylationprotein in THP-1 macrophages. (a) control; (b) palmitic acid 200 μmol/L group; (c) ghrelin 1 nmol/L+ palmitic acid 200 μmol/L group; (d) ghrelin 10 nmol/L+ palmitic acid 200 μmol/L group; (e) ghrelin 100 nmol/L+ palmitic acid 200 μmol/L group. (A) Western blotting electropherogram of TLR4 (96 KD), NF-B p65 (65 KD), and β-actin (42 KD). (B) Acylated ghrelin pretreatment decreased palmitic acid-induced level of TLR4 protein in a dose-dependent in THP-1 macrophages, with values expressed as mean SEM (* versus control; # versus palmitic acid 200 μmol/L group). (C) Acylated ghrelin pretreatment decreased palmitic acid-induced level of NF-B p65 phosphorylationprotein in a dose-dependent in THP-1 macrophages, with values expressed as mean SEM (* versus control; # versus palmitic acid 200 μmol/L group).
