Aberrant Methylation in Promoters of GSTP1, p16, p14, and RASSF1A Genes in Smokers of North India

Table 1

Gene		Annealing temperature (°C)	Primer sequence (5′-3′)	Amplicon size
p-14	MF	55	GTGTTAAAGGGCGGCGTAGC	141
p-14	MR	55	TTTTTGGTGTTAAAGGGTGGTGTAGT
p-14	UF	55	AAAACCCTCACTCGCGACGA	141
p-14	UR	55	CACAAAAACCCTCACTCACAACAA
p-16	MF	56	TTATTAGAGGGTGGGGCGGATCGC	151
p-16	MR	56	TTATTAGAGGGTGGGGTGGATTGT
p-16	UF	56	CCACCTAAATCGACCTCCGACCG	149
p-16	UR	56	CCACCTAAATCAACTCAACCTCCAACCA
RASSF1A	MF	53	GTGTTAACGCGTTGCGTATC	123
RASSF1A	MR	53	TTTGGTTGGAGTGTGTTAATGTG
RASSF1A	UF	53	AACCCCGCGAACTAAAAACGA	123
RASSF1A	UR	53	CAAACCCCACAAACTAAAAACAA
GSTP1	MF	58	TTCGGGGTGTAGCGGTCGT	98
GSTP1	MR	58	GATGTTTGGGGTGTAGTGGTTGTT
GSTP1	MUF	58	GCCCCAATACTAAATCACGACG	108
GSTP1	UR	58	CCACCCCAATACTAAATCACAACA