Degradation Pathway for Eplerenone by Validated Stability Indicating UP-LC Method

Table 3

Regression and precision data.

Parameter	Eplerenone	Imp-1	Imp-2	Imp-3	Imp-4
LOD (%)	0.007	0.017	0.016	0.020	0.018
LOQ (%)	0.02	0.05	0.05	0.05	0.05
Slope (m)	203310	179043	182034	179079	249004
Intercept (C)	472.93	−336.05	733.64	555.31	251.25
Correlation coefficient	0.99903	0.99977	0.99975	0.99976	0.99990
Precision (%RSD)^a	3.0	4.0	2.8	1.9	2.3

Linearity range was LOQ-150% with respect to 1.0 mg/mL eplerenone for impurities; linearity range was 50–150% of eplerenone. ^aSix determinations using LOQ solutions for impurities and eplerenone.