AKT/mTOR as Novel Targets of Polyphenol Piceatannol Possibly Contributing to Inhibition of Proliferation of Cultured Prostate Cancer Cells
Figure 1
Effects of piceatannol on colony formation and cell proliferation in LNCaP, DU145, and PC-3 CaP cells. (a) Control of clonogenicity by piceatannol. Cells were plated in 6-well tissue culture dishes at 2,000โcells/mL and treated with increasing concentrations of piceatannol (0, 1, 5, 10, 25, and 50โM). After 1 week, colonies were stained with 1.25% crystal violet and quantified by measuring the absorbance at 595โnm. Dose-dependent suppression of colony formation by piceatannol was expressed as a percentage of control (set as 100%). Values are expressed as mean ยฑ SD for three separate experiments. The symbol represents statistical significance: . (b) Control of cell growth by piceatannol. Cells were treated with increasing doses of piceatannol (0, 10, and 25โM). The cell numbers were determined at 72โh using a hemocytometer. The bars show dose-dependent suppression of growth, expressed as a percentage of control (set as 100%). Values are expressed as mean ยฑ SD for three experiments. The symbol represents statistical significance: .
