Fluorometric Determination of Drugs Containing α-Methylene Sulfoxide Functional Groups Using 𝑁¹- Methylnicotinamide Chloride as a Fluorogenic Agent

Table 7

Intraday and interday precision data of 1–5 in plasma samples using the proposed method.

Drug	Claimed conc.	Intraday			Interday
		Found conc.*	S.D.	C.V.	Found conc.*	S.D.	C.V.
1 μg/mL	1	0.96	0.28	2.88%	0.96	0.028	2.88%
	2	1.98	0.014	0.71%	2.05	0.035	1.76%
	10	10.1	0.071	0.704%	9.95	0.035	0.35%
	13	13.15	0.106	0.811%	12.8	0.141	1.09%
	15	15.2	0.141	0.936%	14.8	0.141	0.949%
	20	19.9	0.071	0.354%	20.2	0.141	0.704%
	30	29.8	0.141	0.473%	30.2	0.141	0.47%
	40	40.2	0.141	0.353%	39.75	0.177	0.44%
	50	50.1	0.071	0.141%	49.7	0.212	0.43%
2 ng/mL	1200	1219	14	1.11%	1200	0.00	0.0%
	1000	988	9	0.86%	1020	1.4	1.4%
	500	49	7	1.43%	501	1	1.2%
	200	190	7	3.6%	206	4	2.1%
	75	74	1	0.95%	72.5	2	2.4%
3 ng/mL	100	102	1.414	1.4%	101	0.71	0.704%
	300	305	3.536	1.169%	298	1.141	0.47%
	1200	1195	3.536	0.245%	1206	4.25	0.353%
	1300	1301	0.71	0.0544%	1304	2.83	0.217%
	1500	1502	1.414	0.094%	1501	0.71	0.047%
4 ng/mL	100	100.5	0.354	0.353%	101	0.707	0.704%
	300	302	1.1414	0.469%	299	0.707	0.236%
	400	398	1.1414	0.354%	403	2.12	0.528%
	600	597	2.12	0.354%	601	0.707	0.118%
	800	804	2.828	0.353%	794	4.23	0.532%
	1000	1005	3.536	0.353%	995	3.536	0.354%
	1200	1194	4.24	0.354%	1205	3.536	0.294%
	1300	1304	2.828	2.17%	1304	2.828	2.17%
	1400	1408	5.65	0.402%	1396	2.83	0.202%
5 ng/mL	50	49	0.71	1.43%	50.2	0.14	0.28%
	200	199	0.71	0.35%	203	2.12	1.1%
	600	608	5.66	0.93%	595	3.53	0.6%
	800	810	7.1	0.9%	790	7.1	0.88%
	1000	1015	10.6	1.1%	990	7.1	0.71%
	1500	1520	14.1	0.94%	1515	10.6	0.71%
	1800	1785	10.6	0.6%	1781	13.4	0.75%
	2100	2115	10.6	0.5%	2095	3.5	0.16%

*Average of triplicate analyses for each concentration.