Table of Contents
ISRN Chromatography
Volume 2012, Article ID 293830, 5 pages
http://dx.doi.org/10.5402/2012/293830
Research Article

A High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) Qualitative Detection Method Developed for In Vivo Analyses of Toxin Orellanine from the Cortinarius orellanus Fr.—Part II

1Department of Biology, University of Oslo, Blindern, P. O. Box 1066, 0316 Oslo, Norway
2Department of Chemistry, Norwegian University of Science and Technology, 7491 Trondheim, Norway

Received 26 December 2011; Accepted 15 January 2012

Academic Editors: L. Chen, R. Nageswara Rao, T. Richard, and S. Valsecchi

Copyright © 2012 Ilia Brondz and Anton Brondz. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The high-performance liquid chromatography—mass spectrometry (HPLC-MS) qualitative analysis method in negative mode of detection was used to record the presence of orellanine in the stomach contents of rats after ingestion of Cortinarius orellanus. Intoxication with orellanine causes acute renal failure (ARF), which is characterized by sudden loss of the ability of the kidneys to excrete wastes. The detection method presented here can be used as a platform for future development of analytical procedures for detecting orellanine in cases of human intoxication by toadstools. The presence of orellanine in animal stomach contents was analytically recorded and compared with standard orellanine, which was donated by the R&D Department of Jupiter Ltd, Norway. The MS fragmentation described in the literature and the MS fragmentation of standard samples of isolated orellanine verified the presence of orellanine in the stomach contents of laboratory animals. The presence of a diglucoside of orellanine, which was described as part of the toxic orellanine complex by Spiteller et al., in the stomach contents of animals, was also verified using HPLC-MS.