Biological Effects of Phosphate on Fibroblast-Like Synoviocytes
Figure 1
Differential expressions of genes implicated in lipid transfer, phosphate transport, and pathological calcification. hTERT-OA 13A FLS and hTERT-RA 516 FLS were plated in 100 mm plates at 90% confluence. On the second day, medium containing 10% serum was added. Twenty-four hours later, RNA was extracted and examined with semiquantitative RT-PCR for the expression of MSR1, SCARA5, ANKH, ENTPD1, ENPP1, ENPP2, TNSAP, and ADCY3.
