|
| Strain | Genotypea | Source or reference |
|
| XL-1Blue | F′ [proAB+, lacIq, lacZDM15::Tn10 (tetr)], hsdR17, | Laboratory stock |
| supE44, recA1, endA1, gyrA46, thi, relA1, lac |
| DH5α | F−, deoR, endA1, gyrA96, hsdR17(rk-mk+), recA1, | Laboratory stock |
| relA1, supE44, thi-1, D (lacZYA-argF)U169, (f 80lacZ D M15) |
| JE8471 | DE(cya)854, trp, his, ilv | National BioResource Project |
| BW25113 |
F−, rph-1, DE(rhaBAD)568, DE(araBAD)567, | CGSC |
| DElacZ4787, hsdR514, rrnB |
| KR0401 | a derivaative of BW25113 | [11] |
| KR0411 | KR0401yhcB/pBADs-yhcB | This study |
| KR0412 | KR0401 yhcB, λ(araC, -yhcB, bla) | This study |
| KR0413 | KR0412 rodZ::kan | This study |
| KR0422 | KR0401 rodZ, λ(araC, -yhcB, bla) | This study |
| KR0423 | KR0422yhcB::kan | This study |
|
| plasmid | Relevant features or primers used to amplify the indicated gene | |
|
| pKT25 | BACTH vector, KmR | [22] |
| pKnT25 | constructed from pKTop, KmR | This study, [22] |
| pUT18 | BACTH vector, ApR | [22] |
| pUT18c | BACTH vector, ApR | [22] |
| pKnT25-rodA | ctctagaGACGGATAATCCGAATAAAAAAA/ | This study |
| gcggtaccACGCTTTTCGACAACATTTTCC |
| pKnT25-yhcB | yhcB derived from pUT18-yhcB | This study |
| pKT25-cybD | gctctagaGATCGATTATGAAGTATTGCGT/ | This study |
| gcggatccTACAGAGAGTGGGTGTTACGTT |
| pKT25-mreB | gctctagaGTTGAAAAAATTTCGTGGCATG/ | This study |
| cgggatccTCTTCGCTGAACAGGTCGCCG |
| pKT25-mreC | gctctagaGAAGCCAATTTTTAGCCGTGG/ | This study |
| gcggtaccTGCCCTCCCGGCGCACGC |
| pKT25-mreD | gctctagaGGCGAGCTATCGTAGCCAGGGA/ | This study |
| gcggtaccTGCACTGCAAACTGCTGACGGA |
| pKT25-murG | gctctagaGAGTGGTCAAGGAAAGCGATTA/ | This study |
| gcggtaccGCCCGGGCAACCCGGCTCACTT |
| pKT25-rodZ | rodZ derived from pUT18c-rodZ | This study |
| pKT25-yciS | gctctagaGAAATATTTACTCATTTTCTTACTGGT/ | This study |
| gcgaattcTTATTCCTTCGCCGCTGAC |
| pUT18c-rodZ | gctctagaGAATACTGAAGCCACGCAC/ | This study |
| ggaattCTGCGCCGGTGATTGTT |
| pUT18-ftsB | gcctgcag GGGTAAACTAACGCTGCTGTTG/ | This study |
| cgggatcc CGATTGTTTTGCCCCGCAGA |
| pUT18-yhcB | gcctgcag GACCTGGGAATATGCGCTAATT/ | This study |
| cgggatcc TCGCGCTTCGCGCCAGTA |
| pUT18-C1 | gcctgcagGACCTGGGAATATGCGCTAATT/ | This study |
| cgggatccAGGCTGCTGGAGCTTTTTGC |
| pUT18-C2 | gcctgcagGACCTGGGAATATGCGCTAATT/ | This study |
| cgggatccATCTGCACCGGTGCCTGATCGT |
| pUT18-N | gctctagaGCGTTTTGGTAATCGTAAACTAC/ | This study |
| cgggatccTCGCGCTTCGCGCCAGTA |
|
