Table of Contents
ISRN ENDOCRINOLOGY
Volume 2012 (2012), Article ID 340632, 14 pages
http://dx.doi.org/10.5402/2012/340632
Review Article

Polymer-Based Delivery of Glucagon-Like Peptide-1 for the Treatment of Diabetes

Center for Controlled Chemical Delivery, Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT 84112, USA

Received 21 February 2012; Accepted 16 March 2012

Academic Editors: M. Komatsu and J.-F. Tanti

Copyright © 2012 Pyung-Hwan Kim and Sung Wan Kim. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. W. Kim, “Polymeric gene delivery for diabetic treatment,” Diabetes & Metabolism Journal, vol. 35, no. 4, pp. 317–326, 2011. View at Google Scholar
  2. D. J. Drucker and M. A. Nauck, “The incretin system: glucagon-like peptide-1 receptor agonists and dipeptidyl peptidase-4 inhibitors in type 2 diabetes,” Lancet, vol. 368, no. 9548, pp. 1696–1705, 2006. View at Publisher · View at Google Scholar · View at Scopus
  3. L. R. Ranganath, “Incretins: pathophysiological and therapeutic implications of glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1,” Journal of Clinical Pathology, vol. 61, no. 4, pp. 401–409, 2008. View at Publisher · View at Google Scholar · View at Scopus
  4. M. J. Riedel and T. J. Kieffer, “Treatment of diabetes with glucagon-like peptide-1 gene therapy,” Expert Opinion on Biological Therapy, vol. 10, no. 12, pp. 1681–1692, 2010. View at Publisher · View at Google Scholar · View at Scopus
  5. M. E. Doyle and J. M. Egan, “Mechanisms of action of glucagon-like peptide 1 in the pancreas,” Pharmacology and Therapeutics, vol. 113, no. 3, pp. 546–593, 2007. View at Publisher · View at Google Scholar · View at Scopus
  6. E. J. Verspohl, “Novel therapeutics for type 2 diabetes: incretin hormone mimetics (glucagon-like peptide-1 receptor agonists) and dipeptidyl peptidase-4 inhibitors,” Pharmacology and Therapeutics, vol. 124, no. 1, pp. 113–138, 2009. View at Publisher · View at Google Scholar · View at Scopus
  7. A. M. Rowzee, N. X. Cawley, J. A. Chiorini, and G. DiPasquale, “Glucagon-like peptide-1 gene therapy,” Experimental Diabetes Research, vol. 2011, Article ID 601047, 5 pages, 2011. View at Publisher · View at Google Scholar
  8. J. H. Park, Y. E. Earm, and D. K. Song, “Cellular glucose availability and glucagon-like peptide-1,” Progress in Biophysics and Molecular Biology, vol. 107, no. 2, pp. 286–292, 2011. View at Google Scholar
  9. M. J. Riedel, C. W. K. Lee, and T. J. Kieffer, “Engineered glucagon-like peptide-1-producing hepatocytes lower plasma glucose levels in mice,” American Journal of Physiology, Endocrinology and Metabolism, vol. 296, no. 4, pp. E936–E944, 2009. View at Publisher · View at Google Scholar · View at Scopus
  10. P. L. Brubaker, “Minireview: update on incretin biology: focus on glucagon-like peptide-1,” Endocrinology, vol. 151, no. 5, pp. 1984–1989, 2010. View at Publisher · View at Google Scholar · View at Scopus
  11. B. Ahrén, “The future of incretin-based therapy: novel avenues-novel targets,” Diabetes, Obesity and Metabolism, vol. 13, supplement 1, pp. 158–166, 2011. View at Publisher · View at Google Scholar · View at Scopus
  12. L. L. Nielsen, A. A. Young, and D. G. Parkes, “Pharmacology of exenatide (synthetic exendin-4): a potential therapeutic for improved glycemic control of type 2 diabetes,” Regulatory Peptides, vol. 117, no. 2, pp. 77–88, 2004. View at Publisher · View at Google Scholar · View at Scopus
  13. I. Hadjiyanni, L. L. Baggio, P. Poussier, and D. J. Drucker, “Exendin-4 modulates diabetes onset in nonobese diabetic mice,” Endocrinology, vol. 149, no. 3, pp. 1338–1349, 2008. View at Publisher · View at Google Scholar · View at Scopus
  14. Z. Wu, I. Todorov, L. Li, J. R. Bading et al., “In vivo imaging of transplanted islets with 64Cu-DO3A-VS-Cys40-Exendin-4 by targeting GLP-1 receptor,” Bioconjugate Chemistry, vol. 22, no. 8, pp. 1587–1594, 2011. View at Google Scholar
  15. R. Schultz, W. Yan, J. Toppari, A. Völkl, J. Å. Gustafsson, and M. Pelto-Huikko, “Expression of peroxisome proliferator-activated receptor α messenger ribonucleic acid and protein in human and rat testis,” Endocrinology, vol. 140, no. 7, pp. 2968–2975, 1999. View at Google Scholar · View at Scopus
  16. T. Nyström, A. T. Gonon, A. Sjoholm, and J. Pernow, “Glucagon-like peptide-1 relaxes rat conduit arteries via an endothelium-independent mechanism,” Regulatory Peptides, vol. 125, no. 1–3, pp. 173–177, 2005. View at Publisher · View at Google Scholar · View at Scopus
  17. A. K. Bose, M. M. Mocanu, R. D. Carr, C. L. Brand, and D. M. Yellon, “Glucagon-like peptide 1 can directly protect the heart against ischemia/reperfusion injury,” Diabetes, vol. 54, no. 1, pp. 146–151, 2005. View at Publisher · View at Google Scholar · View at Scopus
  18. C. A. Schnabel, M. Wintle, and O. Kolterman, “Metabolic effects of the incretin mimetic exenatide in the treatment of type 2 diabetes,” Vascular Health and Risk Management, vol. 2, no. 1, pp. 69–77, 2006. View at Publisher · View at Google Scholar · View at Scopus
  19. R. Klein, “Hyperglycemia and microvascular and macrovascular disease in diabetes,” Diabetes Care, vol. 18, no. 2, pp. 258–268, 1995. View at Google Scholar · View at Scopus
  20. E. Erdmann, “Diabetes and cardiovascular risk markers,” Current Medical Research and Opinion, Supplement, vol. 21, supplement 1, pp. S21–S28, 2005. View at Publisher · View at Google Scholar · View at Scopus
  21. I. Thrainsdottir, K. Malmberg, A. Olsson, M. Gutniak, and L. Rydén, “Initial experience with GLP-1 treatment on metabolic control and myocardial function in patients with type 2 diabetes mellitus and heart failure,” Diabetes & Vascular Disease Research, vol. 1, no. 1, pp. 40–43, 2004. View at Google Scholar · View at Scopus
  22. L. A. Nikolaidis, S. Mankad, G. G. Sokos et al., “Effects of glucagon-like peptide-1 in patients with acute myocardial infarction and left ventricular dysfunction after successful reperfusion,” Circulation, vol. 109, no. 8, pp. 962–965, 2004. View at Publisher · View at Google Scholar · View at Scopus
  23. B. Gallwitz, “Therapies for the treatment of type 2 diabetes mellitus based on incretin action,” Minerva Endocrinologica, vol. 31, no. 2, pp. 133–147, 2006. View at Google Scholar · View at Scopus
  24. C. F. Deacon, “Therapeutic strategies based on glucagon-like peptide 1,” Diabetes, vol. 53, no. 9, pp. 2181–2189, 2004. View at Publisher · View at Google Scholar · View at Scopus
  25. J. H. Jeong, S. W. Kim, and T. G. Park, “Molecular design of functional polymers for gene therapy,” Progress in Polymer Science, vol. 32, no. 11, pp. 1239–1274, 2007. View at Publisher · View at Google Scholar · View at Scopus
  26. T. G. Park, J. H. Jeong, and S. W. Kim, “Current status of polymeric gene delivery systems,” Advanced Drug Delivery Reviews, vol. 58, no. 4, pp. 467–486, 2006. View at Publisher · View at Google Scholar · View at Scopus
  27. S. O. Han, R. I. Mahato, Y. K. Sung, and S. W. Kim, “Development of biomaterials for gene therapy,” Molecular Therapy, vol. 2, no. 4, pp. 302–317, 2000. View at Publisher · View at Google Scholar · View at Scopus
  28. D. Fischer, T. Bieber, Y. Li, H. P. Elsässer, and T. Kissel, “A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: effect of molecular weight on transfection efficiency and cytotoxicity,” Pharmaceutical Research, vol. 16, no. 8, pp. 1273–1279, 1999. View at Publisher · View at Google Scholar · View at Scopus
  29. K. Kunath, A. Von Harpe, D. Fischer et al., “Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery: comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine,” Journal of Controlled Release, vol. 89, no. 1, pp. 113–125, 2003. View at Publisher · View at Google Scholar · View at Scopus
  30. S. Oh, M. Lee, K. S. Ko, S. Choi, and S. W. Kim, “GLP-1 gene delivery for the treatment of type 2 diabetes,” Molecular Therapy, vol. 7, no. 4, pp. 478–483, 2003. View at Publisher · View at Google Scholar · View at Scopus
  31. S. Choi, S. Oh, M. Lee, and S. W. Kim, “Glucagon-like peptide-1 plasmid construction and delivery for the treatment of type 2 diabetes,” Molecular Therapy, vol. 12, no. 5, pp. 885–891, 2005. View at Publisher · View at Google Scholar · View at Scopus
  32. M. Lee, S. Oh, C. H. Ahn, S. W. Kim, B. D. Rhee, and K. S. Ko, “An efficient GLP-1 expression system using two-step transcription amplification,” Journal of Controlled Release, vol. 115, no. 3, pp. 316–321, 2006. View at Publisher · View at Google Scholar · View at Scopus
  33. H. Y. Nam, Y. Lee, M. Lee et al., “Erythropoietin gene delivery using an arginine-grafted bioreducible polymer system,” Journal of Control Release, vol. 157, no. 3, pp. 437–444, 2012. View at Google Scholar
  34. K. M. Vårum, M. M. Myhr, R. J. N. Hjerde, and O. Smidsrød, “In vitro degradation rates of partially N-acetylated chitosans in human serum,” Carbohydrate Research, vol. 299, no. 1-2, pp. 99–101, 1997. View at Publisher · View at Google Scholar · View at Scopus
  35. H. Akita and H. Harashima, “Nonviral gene delivery,” Contributions to Nephrology, vol. 159, pp. 13–29, 2008. View at Publisher · View at Google Scholar · View at Scopus
  36. O. J. Kwon, E. Kang, S. Kim, and C. O. Yun, “Viral genome DNA/lipoplexes elicit in situ oncolytic viral replication and potent antitumor efficacy via systemic delivery,” Journal of Controlled Release, vol. 155, no. 2, pp. 317–325, 2011. View at Publisher · View at Google Scholar · View at Scopus
  37. N. S. Yew, M. Przybylska, R. J. Ziegler, D. Liu, and S. H. Cheng, “High and sustained transgene expression in vivo from plasmid vectors containing a hybrid ubiquitin promoter,” Molecular Therapy, vol. 4, no. 1, pp. 75–82, 2001. View at Publisher · View at Google Scholar · View at Scopus
  38. Y. S. Lee, S. Shin, T. Shigihara et al., “Glucagon-like peptide-1 gene therapy in obese diabetic mice results in long-term cure of diabetes by improving insulin sensitivity and reducing hepatic gluconeogenesis,” Diabetes, vol. 56, no. 6, pp. 1671–1679, 2007. View at Publisher · View at Google Scholar · View at Scopus
  39. S. L. Samson, E. V. Gonzalez, V. Yechoor, M. Bajaj, K. Oka, and L. Chan, “Gene therapy for diabetes: metabolic effects of helper-dependent adenoviral exendin 4 expression in a diet-induced obesity mouse model,” Molecular Therapy, vol. 16, no. 11, pp. 1805–1812, 2008. View at Publisher · View at Google Scholar · View at Scopus
  40. T. R. Flotte and B. J. Carter, “Adeno-associated virus vectors for gene therapy,” Gene Therapy, vol. 2, no. 6, pp. 357–362, 1995. View at Google Scholar · View at Scopus
  41. M. J. Riedel, D. F. Gaddy, A. Asadi, P. D. Robbins, and T. J. Kieffer, “DsAAV8-mediated expression of glucagon-like peptide-1 in pancreatic beta-cells ameliorates streptozotocin-induced diabetes,” Gene Therapy, vol. 17, no. 2, pp. 171–180, 2010. View at Publisher · View at Google Scholar · View at Scopus
  42. S. H. Choi and H. C. Lee, “Long-term, antidiabetogenic effects of GLP-1 gene therapy using a double-stranded, adeno-associated viral vector,” Gene Therapy, vol. 18, no. 2, pp. 155–163, 2011. View at Publisher · View at Google Scholar · View at Scopus
  43. B. Jeong, Y. H. Bae, D. S. Lee, and S. W. Kim, “Biodegradable block copolymers as injectable drug-delivery systems,” Nature, vol. 388, no. 6645, pp. 860–862, 1997. View at Publisher · View at Google Scholar · View at Scopus
  44. S. Kim and Y. H. Bae, “Long-term insulinotropic activity of glucagon-like peptide-1/polymer conjugate on islet microcapsules,” Tissue Engineering, vol. 10, no. 11-12, pp. 1607–1616, 2004. View at Google Scholar · View at Scopus
  45. P. Caliceti and F. M. Veronese, “Pharmacokinetic and biodistribution properties of poly(ethylene glycol)-protein conjugates,” Advanced Drug Delivery Reviews, vol. 55, no. 10, pp. 1261–1277, 2003. View at Publisher · View at Google Scholar · View at Scopus
  46. S. Lee, Y. S. Youn, S. H. Lee, Y. Byun, and K. C. Lee, “PEGylated glucagon-like peptide-1 displays preserved effects on insulin release in isolated pancreatic islets and improved biological activity in db/db mice,” Diabetologia, vol. 49, no. 7, pp. 1608–1611, 2006. View at Publisher · View at Google Scholar · View at Scopus
  47. S. A. Hjorth, K. Adelhorst, B. B. Pedersen, O. Kirk, and T. W. Schwartz, “Glucagon and glucagon-like peptide 1: selective receptor recognition via distinct peptide epitopes,” Journal of Biological Chemistry, vol. 269, no. 48, pp. 30121–30124, 1994. View at Google Scholar · View at Scopus
  48. C. Q. Pan, J. M. Buxton, S. L. Yung et al., “Design of a long acting peptide functioning as both a glucagon-like peptide-1 receptor agonist and a glucagon receptor antagonist,” Journal of Biological Chemistry, vol. 281, no. 18, pp. 12506–12515, 2006. View at Publisher · View at Google Scholar · View at Scopus
  49. J. G. Kim, L. L. Baggio, D. P. Bridon et al., “Development and characterization of a glucagon-like peptide 1-albumin conjugate the ability to activate the glucagon-like peptide 1 receptor in vivo,” Diabetes, vol. 52, no. 3, pp. 751–759, 2003. View at Publisher · View at Google Scholar · View at Scopus
  50. I. Kim, T. H. Kim, K. Ma et al., “Synthesis and evaluation of human serum albumin-modified exendin-4 conjugate via heterobifunctional polyethylene glycol linkage with protracted hypoglycemic efficacy,” Bioconjugate Chemistry, vol. 21, no. 8, pp. 1513–1519, 2010. View at Publisher · View at Google Scholar · View at Scopus
  51. P. Chollet, M. C. Favrot, A. Hurbin, and J. L. Coll, “Side-effects of a systemic injection of linear polyethylenimine-DNA complexes,” Journal of Gene Medicine, vol. 4, no. 1, pp. 84–91, 2002. View at Publisher · View at Google Scholar · View at Scopus
  52. J. J. Koh, K. S. Ko, M. Lee, S. Han, J. S. Park, and S. W. Kim, “Degradable polymeric carrier for the delivery of IL-10 plasmid DNA to prevent autoimmune insulitis of NOD mice,” Gene Therapy, vol. 7, no. 24, pp. 2099–2104, 2000. View at Publisher · View at Google Scholar · View at Scopus
  53. A. Maheshwari, R. I. Mahato, J. McGregor et al., “Soluble biodegradable polymer-based cytokine gene delivery for cancer treatment,” Molecular Therapy, vol. 2, no. 2, pp. 121–130, 2000. View at Publisher · View at Google Scholar · View at Scopus
  54. T. I. Kim, M. Ou, M. Lee, and S. W. Kim, “Arginine-grafted bioreducible poly(disulfide amine) for gene delivery systems,” Biomaterials, vol. 30, no. 4, pp. 658–664, 2009. View at Publisher · View at Google Scholar · View at Scopus
  55. P. H. Kim, T. I. Kim, J. W. Yockman, S. W. Kim, and C. O. Yun, “The effect of surface modification of adenovirus with an arginine-grafted bioreducible polymer on transduction efficiency and immunogenicity in cancer gene therapy,” Biomaterials, vol. 31, no. 7, pp. 1865–1874, 2010. View at Publisher · View at Google Scholar · View at Scopus
  56. J. Kim, H. Y. Nam, T. I. Kim et al., “Active targeting of RGD-conjugated bioreducible polymer for delivery of oncolytic adenovirus expressing shRNA against IL-8 mRNA,” Biomaterials, vol. 32, no. 22, pp. 5158–5166, 2011. View at Publisher · View at Google Scholar · View at Scopus
  57. P. H. Kim, J. Kim, T. I. Kim et al., “Bioreducible polymer-conjugated oncolytic adenovirus for hepatoma-specific therapy via systemic administration,” Biomaterials, vol. 32, no. 35, pp. 9328–9342, 2011. View at Google Scholar
  58. T. I. Kim, M. Lee, and S. W. Kim, “Efficient GLP-1 gene delivery using two-step transcription amplification plasmid system with a secretion signal peptide and arginine-grafted bioreducible polymer,” Journal of Control Release, vol. 157, no. 2, pp. 243–248, 2012. View at Google Scholar
  59. G. Borchard, “Chitosans for gene delivery,” Advanced Drug Delivery Reviews, vol. 52, no. 2, pp. 145–150, 2001. View at Google Scholar
  60. M. Jean, M. Alameh, M. D. Buschmann, and A. Merzouki, “Effective and safe gene-based delivery of GLP-1 using chitosan/plasmid-DNA therapeutic nanocomplexes in an animal model of type 2 diabetes,” Gene Therapy, vol. 18, pp. 807–816, 2011. View at Publisher · View at Google Scholar · View at Scopus
  61. A. M. Krieg, “CpG motifs in bacterial DNA and their immune effects,” Annual Review of Immunology, vol. 20, pp. 709–760, 2002. View at Publisher · View at Google Scholar · View at Scopus
  62. M. Kumar, Y. Hunag, Y. Glinka, G. J. Prud'Homme, and Q. Wang, “Gene therapy of diabetes using a novel GLP-1/IgG1-Fc fusion construct normalizes glucose levels in db/db mice,” Gene Therapy, vol. 14, no. 2, pp. 162–172, 2007. View at Publisher · View at Google Scholar · View at Scopus
  63. C. Kurschner, L. Ozmen, G. Garotta, and Z. Dembic, “IFN-γ receptor-Ig fusion proteins: half-life, immunogenicity, and in vivo activity,” Journal of Immunology, vol. 149, no. 12, pp. 4096–4100, 1992. View at Google Scholar · View at Scopus
  64. G. J. Prud'Homme and Y. Chang, “Prevention of autoimmune diabetes by intramuscular gene therapy with a nonviral vector encoding an inteferon-gamma receptor/IgG1 fusion protein,” Gene Therapy, vol. 6, no. 5, pp. 771–777, 1999. View at Publisher · View at Google Scholar · View at Scopus
  65. E. Kang and C. O. Yun, “Current advances in adenovirus nanocomplexes: more specificity and less immunogenicity,” BMB Reports, vol. 43, no. 12, pp. 781–788, 2010. View at Publisher · View at Google Scholar · View at Scopus
  66. R. Cattaneo, T. Miest, E. V. Shashkova, and M. A. Barry, “Reprogrammed viruses as cancer therapeutics: targeted, armed and shielded,” Nature Reviews Microbiology, vol. 6, no. 7, pp. 529–540, 2008. View at Publisher · View at Google Scholar · View at Scopus
  67. R. Singh and K. Kostarelos, “Designer adenoviruses for nanomedicine and nanodiagnostics,” Trends in Biotechnology, vol. 27, no. 4, pp. 220–229, 2009. View at Publisher · View at Google Scholar · View at Scopus
  68. N. Koizumi, H. Mizuguchi, F. Sakurai, T. Yamaguchi, Y. Watanabe, and T. Hayakawa, “Reduction of Natural Adenovirus Tropism to Mouse Liver by Fiber-Shaft Exchange in Combination with both CAR- and αv Integrin-Binding Ablation,” Journal of Virology, vol. 77, no. 24, pp. 13062–13072, 2003. View at Publisher · View at Google Scholar · View at Scopus
  69. G. B. Parsons, D. W. Souza, H. Wu et al., “Ectopic expression of glucagon-like peptide 1 for gene therapy of type II diabetes,” Gene Therapy, vol. 14, no. 1, pp. 38–48, 2007. View at Publisher · View at Google Scholar · View at Scopus
  70. M. J. Liu, S. Shin, N. Li et al., “Prolonged remission of diabetes by regeneration of β cells in diabetic mice treated with recombinant adenoviral vector expressing glucagon-like peptide-1,” Molecular Therapy, vol. 15, no. 1, pp. 86–93, 2007. View at Publisher · View at Google Scholar · View at Scopus
  71. Y. Lee, M. K. Kwon, E. S. Kang et al., “Adenoviral vector-mediated glucagon-like peptide 1 gene therapy improves glucose homeostasis in Zucker diabetic fatty rats,” Journal of Gene Medicine, vol. 10, no. 3, pp. 260–268, 2008. View at Publisher · View at Google Scholar · View at Scopus
  72. R. M. Kotin, M. Siniscalco, R. J. Samulski et al., “Site-specific integration by adeno-associated virus,” Proceedings of the National Academy of Sciences of the United States of America, vol. 87, no. 6, pp. 2211–2215, 1990. View at Google Scholar · View at Scopus
  73. R. J. Samulski, X. Zhu, X. Xiao et al., “Targeted integration of adeno-associated virus (AAV) into human chromosome 19,” The EMBO Journal, vol. 10, no. 12, pp. 3941–3950, 1991. View at Google Scholar · View at Scopus
  74. S. Choi, M. Baudys, and W. K. Sung, “Control of blood glucose by novel GLP-1 delivery using biodegradable triblock copolymer of PLGA-PEG-PLGA in type 2 diabetic rats,” Pharmaceutical Research, vol. 21, no. 5, pp. 827–831, 2004. View at Publisher · View at Google Scholar · View at Scopus
  75. H. Gappa, M. Baudyš, J. J. Koh, S. W. Kim, and Y. H. Bae, “The effect of zinc-crystallized glucagon-like peptide-1 on insulin secretion of macroencapsulated pancreatic islets,” Tissue Engineering, vol. 7, no. 1, pp. 35–44, 2001. View at Publisher · View at Google Scholar · View at Scopus
  76. H. Kim, H. Park, J. Lee et al., “Highly porous large poly(lactic-co-glycolic acid) microspheres adsorbed with palmityl-acylated exendin-4 as a long-acting inhalation system for treating diabetes,” Biomaterials, vol. 32, no. 6, pp. 1685–1693, 2011. View at Publisher · View at Google Scholar · View at Scopus
  77. J. Panyam and V. Labhasetwar, “Biodegradable nanoparticles for drug and gene delivery to cells and tissue,” Advanced Drug Delivery Reviews, vol. 55, no. 3, pp. 329–347, 2003. View at Publisher · View at Google Scholar · View at Scopus
  78. M. J. Roberts, M. D. Bentley, and J. M. Harris, “Chemistry for peptide and protein PEGylation,” Advanced Drug Delivery Reviews, vol. 54, no. 4, pp. 459–476, 2002. View at Publisher · View at Google Scholar · View at Scopus
  79. S. H. Lee, S. Lee, S. Y. Yu et al., “Synthesis, characterization, and pharmacokinetic studies of PEGylated glucagon-like peptide-1,” Bioconjugate Chemistry, vol. 16, no. 2, pp. 377–382, 2005. View at Publisher · View at Google Scholar · View at Scopus
  80. Y. Li, X. Li, X. Zheng, L. Tang, W. Xu, and M. Gong, “Disulfide bond prolongs the half-life of therapeutic peptide-GLP-1,” Peptides, vol. 32, no. 7, pp. 1400–1407, 2011. View at Publisher · View at Google Scholar · View at Scopus
  81. Y. Li, X. Zheng, L. Tang, W. Xu, and M. Gong, “GLP-1 analogs containing disulfide bond exhibited prolonged half-life in vivo than GLP-1,” Peptides, vol. 32, no. 6, pp. 1303–1312, 2011. View at Publisher · View at Google Scholar · View at Scopus
  82. L. Ranganath and L. Morgan, “An osmotic stimulus-mediating glucagon-like peptide-1 (7-36 amide) (GLP-1) secretion in acarbose-induced sucrose malabsorption?” Nutrition, vol. 16, no. 1, pp. 64–65, 2000. View at Publisher · View at Google Scholar · View at Scopus
  83. A. Lee, P. Patrick, J. Wishart, M. Horowitz, and J. E. Morley, “The effects of miglitol on glucagon-like peptide-1 secretion and appetite sensations in obese type 2 diabetics,” Diabetes, Obesity and Metabolism, vol. 4, no. 5, pp. 329–335, 2002. View at Publisher · View at Google Scholar · View at Scopus