Porphyromonas gingivalis Fimbria-Induced Expression of Inflammatory Cytokines and Cyclooxygenase-2 in Mouse Macrophages and Its Inhibition by the Bioactive Compounds Fibronectin and Melatonin

Figure 1

Binding of fibronectin in saliva to P. gingivalis fimbrillin. The purified fimbriae (10 μg/mL) were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in 12.5% polyacrylamide gel, and then the separated samples were transferred to a PVDF membrane. The membrane was then treated for 2 h at 37°C with saliva from healthy subjects. Salivary FN that became bound to the fimbriae was analyzed by overlay Western Blotting with anti-FN antibody and visualized using horseradish-peroxidase (HRP-) conjugated goat anti-rabbit IgG. Three independent experiments were performed, and similar results were obtained (Copyright Wiley-Blackwell).