Effect of Chronic L-Dopa or Melatonin Treatments after Dopamine Deafferentation in Rats: Dyskinesia, Motor Performance, and Cytological Analysis
The present study examines the ability of melatonin to protect striatal dopaminergic loss induced by 6-OHDA in a rat model of Parkinson's disease, comparing the results with L-DOPA-treated rats. The drugs were administered orally daily for a month, their therapeutic or dyskinetic effects were assessed by means of abnormal involuntary movements (AIMs) and stepping ability. At the cellular level, the response was evaluated using tyrosine hydroxylase immunoreactivity and striatal ultrastructural changes to compare between L-DOPA-induced AIMs and Melatonin-treated rats. Our findings demonstrated that chronic oral administration of Melatonin improved the alterations caused by the neurotoxin 6-OHDA. Melatonin-treated animals perform better in the motor tasks and had no dyskinetic alterations compared to L-DOPA-treated group. At the cellular level, we found that Melatonin-treated rats showed more TH-positive neurons and their striatal ultrastructure was well preserved. Thus, Melatonin is a useful treatment to delay the cellular and behavioral alterations observed in Parkinson's disease.
Parkinson disease (PD) is an age-related disorder characterized by a progressive degeneration of dopaminergic (DAergic) neurons of the substantia nigra pars compacta (SNc). The etiology of DAergic neurons death is not known. However, reported data suggest oxidative stress as the probable candidate to mediate in the original unknown cause. Studies on patients’ brains have given evidences in support of this hypothesis. Levels of reduced glutathione (GSH) are low , while the level of the antioxidant enzyme manganese superoxide dismutase (SOD) is high and is not paralleled by a rise in glutathione peroxidase (GPX) . Iron level increase has also been reported . Since iron is able to catalyze the Fenton reaction, this implies hydroxyl radical (•OH) formation. Radical damage has been demonstrated in lipids , proteins, and nucleic acids  of the SNc of Parkinsonian patients. Thus, generation of reactive oxygen species (ROS) caused by oxidative stress, together with a relative lack of antioxidant defenses in the basal ganglia and nigrostriatal DAergic pathway, is commonly considered the final cause of neuronal death [6, 7].
L-DOPA (LD) is the drug of election in PD therapy. LD is converted by neuronal aromatic L-amino acid decarboxylase into dopamine (DA), reestablishing DA levels in surviving neurons, which, despite the treatment, continue to die [8, 9], and whereas LD treatment is very successful in the early stages of PD, it does not prevent disease progression, and in the long term there are undesirable side effects, such as the development of dyskinesias [10–12]. Dyskinesia interferes with physiological motor activity and constitutes a serious challenge to the management of PD (for review see Obeso et al. ). Previous studies have investigated the reasons for such long-term problems. Some suggested mechanism describes LD to elicit oxidative damage perpetuating the cell death [14–17], and, after MPTP infusion, it seems that LD generates 6-hydroxydopamine (6-OHDA) in the mouse striatum producing more ROS formation  enhancing iron-induced lipid peroxidation both in vitro and in vivo in the rat striatum . Other evidences suggest that motor complications associated with sustained LD therapy are the consequence of irregular and intermittent delivery of LD to the brain, resulting in nonphysiologic pulsatile stimulation of striatal DA receptors . Thus, the short half-life of immediate-release LD formulations is thought to be the key factor in the pathogenesis of motor complications . Novel treatments for PD will be successful to the extent that they can either retard or prevent the development of these complications.
It has been recently established that DAergic and serotoninergic nuclei in the brain are overloaded with DA following acute, subacute, or chronic administration of LD , and the increased DA in the former nuclei can cause production of 6-OHDA in the brain . Therefore, we hypothesized that Parkinsonian neurotoxins that generate •OH in a DA-enriched environment would be conducive to oxidative stress production, and melatonin may act as a potent free radical scavenger and protect against ROS formation and the resulting DAergic neuronal death.
Melatonin is an indolamine first reported in 1993 by Tan et al.  as an efficient endogenous antioxidant. Melatonin possesses several unique advantages. First, its solubility in both lipids and water allows it to be easily distributed into the cell. Second, its ability to cross the brain-blood barrier allows melatonin to enter the central nervous system .
Melatonin has been proven to protect neuronal cells from neurotoxin-induced damage in a wide range of neuronal culture systems serving as experimental models for the study of PD (for review see ). In vivo experiments are however scarce and have almost always been done in acute experimental models of the disease. These acute studies show protective effects of melatonin in both the striatum DAergic axons  and the midbrain neurons .
There is considerable evidence that pharmacological doses of melatonin are neuroprotective in diverse models of neurodegeneration including PD. However, there is limited information about its effects on the initial stages of neurodegeneration.
Therefore, the present study was undertaken to determine the ability of melatonin to protect striatal DAergic loss induced by 6-OHDA in a rat model of PD, comparing the results with LD-treated rats. The drugs were administered four days following lesioning daily for a month at doses sufficient to improve physiological motor performance, and their therapeutic or dyskinetic effects were assessed using measures of abnormal involuntary movements (AIMs), skilled forelimb use, and stepping ability. At the cellular level, the response to the drugs was evaluated using tyrosine hydroxylase (TH) immunoreactivity and striatal ultrastructural changes to compare between LD-induced AIMs and melatonin-treated rats.
2. Experimental Procedures
The experiments were carried out in 24 male Wistar rats weighing 180–200 g at the beginning of the study. The rats were individually housed in hanging plastic cages under controlled light conditions (12 h light/h dark regime) and fed with Purina Rat Chow and water ad libitum. Body weight was recorded daily. The experimental protocol was conduced in accordance with the Animal Act of 1986 for Scientific Procedures. All efforts were made to minimize the number of animals used and their suffering.
2.1. Beam-Walking Task
Before 6-OHDA surgery, all animals were trained for one week to cross two narrow wooden beams (6 and 12 mm) into a safe platform. The beams measured 2 meters long and were elevated to a height of 1 m above the floor with wooden supports with 15° inclination. Each test session consisted of four trials in which latency to cross the beam was recorded (we established a maximum range of 120 seconds; if the animal did not cross at that time, the activity was terminated and we assigned the value of 120 seconds for that evaluation). Five trials were averaged to give a mean latency, and testing was done every week after 6-OHDA lesion. Training and testing were performed during the lighted portion of the cycle, at the same hour every time. Two observers blind to the rat treatment or control status perform all behavioral assessments.
2.2. Stereotactic Surgery and Treatments
The rats were anesthetized with sodium pentobarbitone (35 mg/kg i.p.) and placed in a stereotaxic apparatus. The rats were injected with 4 μL of a saline solution containing 8 μg of 6-OHDA (Sigma Chemical, USA) and 0.2 mg of ascorbic acid into the right medial forebrain bundle (), and sham lesion was made with vehicle ( (control group)). The injections were given over a 4 min period with a Hamilton syringe attached to a glass micropipette with a tip diameter of 20–50 μm. The stereotaxic coordinates were as follows: mm anterior of the ear bar; mm lateral of bregma; mm vertical of dura (according to ). After recovery from the anesthesia, the animals were returned to their home cages. Apomorphine (Sigma Chemical, USA; 0.25 mg/kg i.p.) induced rotational behavior was tested two days after lesioning. Only those animals exhibiting more than 200 complete turns in a 30 min period were used . Two days after the test, six experimental animals were treated with 7.5-mg/kg L-DOPA (Sinemet (Carbidopa-L-DOPA 25/250)), and 6 were treated with 10 mg/kg melatonin (Sigma Chemical, USA). The drugs were dissolved in 10 mL distilled water and given orally with an insulin syringe for a month. The other six lesioned rats without treatment, as well as the control animals, were kept for the same time. The motor performance was evaluated weekly.
2.3. AIMs Rating
LD-induced AIMs were scored at day 30 according to a rat dyskinesia scale [29–31]. Rats were placed individually in transparent plastic cages and observed every 20th min, from 20 min before to 180 min after giving LD (10 monitoring periods of 1 min each). Four subtypes of AIMs were classified according to their topographic distribution as locomotive, axial, forelimb, or orolingual (for details see Figure 4). Enhanced manifestations of otherwise normal behaviors, such as rearing, sniffing, grooming, and gnawing, were not included in the rating. AIM severity was assessed using the published method of Cenci et al. , Lee et al. , and Lundblad et al. , which assigns a score from 0 to 4 to each of the four AIM subtypes listed above according to the proportion of time/monitoring period during which the AIM is present. Borderline scores, such as 0.5, 1.5, 2.5, and 3.5, were allowed in order to increase the sensitivity of the evaluation.
2.4. Video Recording
Performance during beam walking test and AIM analysis was video recorded using a Panasonic camcorder (SDR-H80 model). Representative still frames were captured from digital video recordings with the video editing software Final Cut Pro. Pictures were cropped and adjusted for color and brightness contrast in Adobe Photoshop but were not altered in any other way.
2.5. Tissue Preparation
All rats were perfused under sodium pentobarbital anesthesia immediately after the one-month treatments via aorta, with saline solution followed by fixative containing 0.2% glutaraldehyde and 4% paraformaldehyde in 0.1 M phosphate buffer (PB). The brains were removed and placed in fixative solution for 1 hour.
2.6. TH Immunocytochemistry
Coronal sections (50 μm) were obtained on a vibrating microtome through the mesencephalon for immunocytochemistry. Tyrosine hydroxylase (Chemicon International, Inc., CA, USA, 1 : 1000) immunostaining with the ABC detection method (Vector Lab MI, USA) was performed for light microscope analysis. The analysis was conducted with a computer-assisted system (Image-Pro Plus, Media Cybernetics, L.P. Del Mar, CA, USA) connected by a CCD camera to Optiphot 2 microscope (Nikon, Japan). The number of TH-positive neurons was counted in 1500 μm2 from 7 SNc sections of each animal .
2.7. Electron Microscopy
Fragments from ipsilateral and contralateral striata were carefully taken. After washing in PB, the fragments were treated for 60 minutes with 1% osmium tetroxide in PB, washed for 30 minutes in PB, dehydrated with graded ethanol, and flat-embedded in araldite. Ultrathin sections were collected, counterstained with uranyl acetate and lead citrate, and examined in a JEOL 100CX-II electron microscope.
2.8. Ultrastructural Analysis
Synapses were defined by the presence of a clear postsynaptic density facing at least three presynaptic vesicles. Ultrastructural analysis was performed in 50 randomly selected synaptic endings per striatum. In each synaptic bouton, we observed all its membrane and organelle features, and we measured the following.(i)The diameter of the presynaptic bouton using two axes, which were perpendicular to each other and intersected at the center of the synaptic terminal (Figure 1); the diameter was measured directly from the electron microscope screen with a grid placed inside the eyepiece .(ii)The number of dendritic spines or dendrites as postsynaptic targets.(iii)Perforated synapses were identified on micrographs of serial sections and defined by the presence of a discontinuity in the postsynaptic density . The number of perforated synapses was determined considering the following characteristics according to Calverley and Jones : the site of the perforation projects into the presynaptic terminal, the active zone has one or more negatively curved components, which are separated by a central region of the active zone that projects into the presynaptic terminal, and the presynaptic density is in close association to the spine apparatus or an extension of it.
To minimize subjectivity, classification was carried out blind by at least two experimenters, and if distinction was unclear, the synapse was not included in the quantification.
2.9. Statistical Analysis
One-way ANOVA was used to analyze the number of TH-immunoreactive cells and behavioral data. Group differences were considered statistically significant at . When appropriate, post hoc comparisons were made with the Tukey test. All analyses were conducted with GraphPad Prism 5 Software.
After 1 month, neither clinical alterations nor significant weight changes were detected in the experimental animals compared with controls.
3.1. Beam-Walking Test
Figure 2 illustrates the mean numbers of total time to cross the beam. In both beams 6 mm and 12 mm thick, control animals showed no significant difference in the time they took to traverse the beam. In contrast, 6-OHDA group, the animals had a very similar behavior in both beams, taking longer to traverse them, becoming more evident at more time after lesion; moreover lesioned animals with no treatment had some motor features such as freezing behavior, and, when moving, the rats were dedicated mainly to explore the beam rather to cross it, the rats steps were very slow compared to the control rats and several 6-OHDA-lesioned animals could not transverse the beam in the established time. On the other hand, the 6-OHDA-lesioned + LD treatment group since the beginning of treatment showed significant recovery of motor behavior until 21 days; these animals took less time to traverse the beams compared to the 6-OHDA-lesioned group and showed even better times than control group, suggesting hyperactivity, but afterwards had a significant increase in the time to cross and a significant potentiation of freeze time (data not shown) compared to control and 6-OHDA-lesioned + melatonin-treated rats. The 6-OHDA-lesioned + melatonin group at 7 and 14 days evaluation showed values very similar to 6-OHDA group without treatment, but, since 21 days, this group demonstrated an evident motor recovery.
3.2. Abnormal Involuntary Movements (AIMs)
3.2.1. Time Course and Overall Incidence AIMs
In order to get an overview of the development of dyskinesia in the different groups, we carried out the summation of all subtypes of AIMs (axial + locomotive + limb + orolingual). As shown in Figure 3(a), repeated measures ANOVA revealed significant overall differences between 6-OHDA-lesioned and melatonin-treated groups comparing to LD-treated group. Figure 3(b) (treatment day 30) depicts the temporal manifestation of AIMs after a dose of LD or melatonin resembling the time course of peak-dose dyskinesia in PD [36, 37]. Therefore, AIM severity progressively increased during the first 20 min posttreatment, continued elevated for an additional 60 min, and then gradually returned to baseline between 100 and 160 min posttreatment.
3.2.2. Representation of AIM Subtypes
According to Cenci et al.  and Lee et al. , the animals were evaluated on four different topographic subtypes of AIMs, which are represented in Figure 4. Different AIM subtypes were mainly characterized among the LD-treated group (Figure 5). The 6-OHDA-lesioned animals and animals treated with melatonin showed no locomotive AIMs, contrasting to the animals treated with LD that manifest this behavior (Figure 5(a)). Animals treated with LD showed severely abnormal involuntary movements affecting the muscles of the neck and trunk, observed by the increase in axial AIMs score, unlike the 6-OHDA-lesioned animals and melatonin-treated group where these muscles were not affected (Figure 5(c)). Finally, the orolingual (Figure 5(b)) and forelimb AIMs (Figure 5(d)) were observed in all experimental groups; nevertheless, the score of the 6-OHDA-lesioned animals and melatonin-treated group were very small and showed no significant differences compared to control animals. However, animals treated with LD showed a significant increase in these dyskinetic movements.
3.3. TH Immunocytochemistry
The number of TH-positive neurons in the control group, both the ipsi and contralateral SNc remained unchanged ( and , resp.). In contrast, we found an important loss of TH-positive neurons in the SNc of 6-OHDA lesioned animals in both ipsilateral () and contralateral () SNc compared to controls as shown in Figures 6 and 7; likewise, LD-treated rats ( and contralateral and ipsilateral, resp.) and melatonin-treated rats ( contralateral and ipsilateral SNc) show significant loss of TH-positive cells; however, melatonin-treated animals exhibited less neuronal loss compared to the group treated with LD.
3.4. Electron Microscopy
3.4.1. Diameters of Synaptic Endings
Control rats did not show any differences between both striata synaptic endings diameter and neuropile alterations after sham surgery (Figures 8 and 9(A)). As shown in Figure 8, the synaptic endings of the control group major axis presented an average of nm on the contralateral striatum and nm on the ipsilateral one; the minor axis mean was nm on the contralateral and μm2 on the ipsilateral striatum. The 6-OHDA-lesioned group showed an evident increase in the size of synaptic boutons ( and nm minor and major axis, respectively, of the ipsilateral striatum); the same pattern was observed in the 6-OHDA + LD-treated group ( and nm minor axis and major axis of the ipsilateral side, resp.); there were statistically significant differences in both groups comparing to control group (Figure 8). 6-OHDA + melatonin-treated group showed fewer presynaptic buttons with edema ( nm and nm minor and major axis of the ipsilateral side, resp.), showing statistically significant differences comparing to 6-OHDA + LD and lesioned untreated groups. The contralateral (nonlesioned side) of all experimental groups (6-OHDA, 6-OHDA + L-DOPA and 6-OHDA + melatonin) showed no statistically significant differences compared to control group (Figure 8).
3.4.2. Postsynaptic Target
When analyzing the postsynaptic structure (spine or dendrite) with which synaptic contact was established, we note that in control group prevailed synaptic contacts with dendritic spines (Figures 9 and 10) show an average of for the contralateral side and for the ipsilateral one, unlike 6-OHDA group () and 6-OHDA + LD-treated group (), where there was an important decrease in the number of synaptic contacts established with dendritic spines in ipsilateral striatum (Figures 9(B), 9(C), and 10); there were significant differences comparing to control group and the group treated with melatonin; the latter presented similar number of synaptic contacts with dendritic spines than the control group (Figures 9(D) and 10).
3.4.3. Perforated Synapses
Figure 11 depicts the number of perforated synaptic contacts in the different groups; the control group had a mean of contralateral and in the ipsilateral striata, in contrast; all experimental groups showed an increase in the number of perforated contacts in both striata, becoming more evident in the ipsilateral side; we found significant differences compared to the control group (Figures 9(D) and 11).
The findings of the present study demonstrated that chronic oral administration of melatonin corrected a hemi-Parkinson’s condition in rats caused by intramedial forebrain bundle application of the neurotoxin 6-OHDA. melatonin-treated animals perform better in the motor tasks and had no dyskinetic alterations compared to LD-treated rats. At the cellular level, we found that melatonin-treated rats showed more TH-positive neurons and their striatal ultrastructure was well preserved, which probably led to the functional recovery.
4.1. Motor Behavior
Our results show that 6-OHDA-lesioned animals displayed decreased motor coordination, becoming more evident over time following lesion. This finding is consistent with the results of Allbut and Henderson , who demonstrate that rats unilaterally lesioned with 6-OHDA in the medial forebrain bundle presented motor behavior alterations when evaluated on a beam-walking test; they observed that the time the rats took to traverse the beam was drastically increased compared to control group; animals also showed rigidity and restraint in their movements when walking the beam. Some rats roamed the beam and stopped and then restarted or just stand still, which is analogous to the behaviors observed in patients with PD. Also, Truong et al.  conducted a study in rats unilaterally lesioned with 6-OHDA at different doses and subsequently evaluated motor behavior in a wooden beam. The animals showed a travel time of about 1 minute at 2 and 4 weeks after lesion, while the control group showed a travel time of 0.2 minutes, concluding that changes in motor performance are correlated with the loss of DAergic cells.
As described in our results, animal treated with LD showed improvement to traverse the beam until 21 days, but after 28 days the treatment was no longer able to reduce the motor alterations induced by 6-OHDA-lesion Our results also show that animals with DA denervation reproduce dyskinetic motor effects when treated with therapeutical doses of LD. As we mentioned previously, LD treatment is the most effective drug for PD treatment, since no other drug matches its ability to suppress the symptoms. However, after chronic treatment, 30 to 80% of patients develop side effects such as dyskinesias that become more disabling than the disease itself . In this regard Rajput et al.  stand out in their review that patients who received LD, about two-thirds, had benefits within the first three to six months of treatment, but after a while show a clear deterioration accompanied by a constant increased complications, such as changes in threshold and AIMs . It has also been reported that patients who develop dyskinesias due to prolonged use of LD are characterized by involuntary and uncontrollable chaotic movements of the mouth, cheeks, arms, and legs [43, 44]; these dyskinesias may be considered a negative reaction of brain plasticity in response to the time of disease progression and prolonged use of LD [44, 45].
The mechanism by which LD induces motor complications has not been established, but several theories have been proposed: (A) the fluctuations may occur due to deficiencies in the synthesis and storage of DA in DAergic terminals ; (B) the DA transporter system, which represents a major mechanism by which DA is removed from the synapse (which is a crucial element involved in the regulation of DAergic transmission) , is altered by prolonged LD treatment ; (C) it seems that LD produce oxidative damage perpetuating cell death [14–17] enhancing iron-induced lipid peroxidation . Other evidences suggest that motor complications associated with sustained LD therapy are a result of irregular and intermittent delivery of LD to the brain, resulting in nonphysiologic pulsatile stimulation of striatal dopamine receptors .
On the other hand, animals treated with melatonin showed a motor performance very similar to 6-OHDA-lesioned group at 7 to 14 days; afterwards the animals improved; this became more evident at 28 days after lesion. We consider that melatonin-treated animals probably do not improve their motor behavior at the start of treatment, as the neurotoxin is highly aggressive and causes some cells no longer functional (i.e., discontinue producing DA) without necessarily inducing death , and therefore display a motor imbalance very similar to 6-OHDA-lesioned group; thus it is feasible to think that melatonin treatment trigger different signaling pathways to enhance the mechanism against ROS produced by 6-OHDA; so after some time it is possible that the cell is able to recover its DAergic phenotype, producing a modulatory effect reflected in functional recovery. It has also been reported that elevated ROS participate in 6-OHDA neurotoxicity; this has been evidenced by the reduction in brain GSH levels and in the loss of SOD activity . Melatonin stimulates antioxidant enzymes such as SOD, GPX, and glutathione reductase . Singh et al.  demonstrated that systemic administration of melatonin protected striatal DAergic neurons against 6-OHDA neurotoxicity in the rat. The effect was accompanied by a significant recovery in motor behavior tests.
In this regard Singh et al.  have reported that animals pretreated systemically with melatonin and subsequently lesioned with 6-OHDA and treated with melatonin for a period of 7 days showed a decrease in the number of apomorphine-induced rotations, improved posture, and slowness of movement compared to 6-OHDA-lesioned treated with vehicle solution group. These results demonstrate that melatonin treatment may have beneficial effects for the treatment of PD. Furthermore, Hamdi  conducted a study in animals where he administered melatonin in drinking water and found that the striatum had a higher affinity to D2 receptor, while the number of receptors did not change. The mechanism by which melatonin increases the affinity of D2 receptors is unknown, but the author suggests that this effect may be produced through conformational changes in the receptor-binding site. This mechanism may involve a functional alteration by a direct or indirect effect of melatonin on one or more levels that is, gene expression and receptor proteins synthesis; thus melatonin may represent a modulatory influence on the DAergic system .
4.2. TH Immunocytochemistry
We observed a severe decrease of TH-positive cells after depleting the nigrostriatal pathway; our results agree with Damier et al.  who found that PD patients showed a reduction of DAergic neurons up to 95% depending on the time of clinical evolution. Likewise, it has been demonstrated that the medial forebrain bundle unilateral lesion reduces 98% the SNc ipsilateral number of TH-immunoreactive neurons [38, 55]. We found that 6-OHDA-lesioned and 6-OHDA + LD-treated groups displayed a very similar pattern, showing a dramatic loss of TH-immunoreactive neurons. The cell loss after LD treatment may have been because LD is converted to DA by the enzyme AADC and thus raises the levels of DA in the striatum , this in consequence may act as a cell death perpetrator, since there is evidence that suggests that large amounts of DA, in addition to serving as a neurotransmitter, can act as a neurotoxin. It has been found in culture cells exposed to high levels of DA that DA is able to produce apoptotic neuronal death , decrease levels of GSH, and increase intracellular Ca2+; these effects of DA oxidation in cellular physiology in vivo may occur under conditions of oxidative stress, increasing the vulnerability of DAergic neurons to degeneration . Furthermore, Maharaj et al.  demonstrate that LD might accelerate the rate of SNc degeneration because it undergoes oxidative metabolism to form 6-OHDA.
About the data obtained after melatonin treatment, we observed that this indolamine partially prevents SNc DAergic cell damage produced by 6-OHDA lesion. 6-OHDA toxicity is based on direct inhibition of complex I of the electron transport chain in the mitochondria . Inhibition of this complex has also been reported in the SNc of patients suffering PD. This inhibition causes energy depletion and increases free radical concentration in the mitochondria . In the present work, for melatonin-treated group, although the cell percentage decreases (comparing ipsilateral to contralateral sides), the loss was less severe than that detected in 6-OHDA-lesioned and LD-treated rats. The antioxidant effects of melatonin and its protective effects against the uncoupling of the electron transport chain of several toxins in the mitochondria have been well summarized by Acuña-Castroviejo et al. . These data urge us to undertake further studies based on this hypothesis. In vivo studies with melatonin in experimental models of PD are however scarce. Acuña-Castroviejo et al.  found that melatonin prevented an increase in lipid peroxidation and a decrease in TH immunoreactivity in the striatum after a single dose of MPTP, arguing that melatonin was able to prevent the damage caused by this drug in the striatal dopaminergic axons. Ortiz et al.  reported, using DNA electrophoresis, apoptosis of midbrain neurons induced by a single dose of MPTP. Melatonin also prevented both cell damage and DNA fragmentation. Also, melatonin was able to counteract the decrease in striatal TH immunoreactivity and the loss of complex I activity produced in rats after acute 6-OHDA administration [62, 63]. Similarly, Kim et al.  showed that melatonin treatment rescues nigrostriatal dopaminergic neurons from cell death in 6-OHDA-lesioned rats suggesting that this beneficial effect was a consequence of the potent antioxidative action of melatonin. Thus, melatonin has been reported to protect against 6-OHDA , MPTP , and MPP+ lesions . However, it should be emphasized that these melatonin-induced effects have been traditionally discussed in the context of the efficient free radical scavenger and antioxidant properties of this pineal hormone (for a review see Reiter ). Nevertheless, our data show a stimulating effect of melatonin over TH-immunoreactivity suggesting a role over the nigrostriatal DAergic system. In fact, melatonin has been shown to regulate striatal dopaminergic activity and block LD-induced dyskinesias . As we mentioned above, it is therefore plausible to assume a role for melatonin as a neuromodulator in the nigrostriatal DAergic system ; however, further analyses are needed to clarify this point.
4.3. Electron Microscopy
The ultrastructural analysis of the striatum neuropile after unilateral 6-OHDA lesion of the medial forebrain bundle of rats and after LD treatment revealed that this neurotoxin induces derangement of the ipsilateral striatum neuropile characterized by (1) edema of the presynaptic buttons, (2) changes on the postsynaptic targets, and (3) increase in the number of perforated synapses, alterations that improved with melatonin treatment.
The present analysis confirms previous observations [33, 68–70], concerning to the fact that DA depletion of the nigrostriatal pathway causes an increment in the size of synaptic buttons. The authors assume that this swelling is due to an inherent degenerative process caused by 6-OHDA lesion, because they saw this increment in size in almost all synaptic endings that were measured. Here, we observe that melatonin treatment prevented synaptic ending swelling. Biochemical abnormalities relevant to the pathogenesis of PD include mitochondrial dysfunction, free radical-mediated damage, excitotoxicity, and inflammation [71, 72]. About the anti-inflammatory effects of melatonin, the most important feature is its inhibition of iNOS expression . melatonin administration prevented mitochondrial iNOS induction in sepsis and in MPTP-treated mice, avoiding respiratory chain dysfunction and preserving ATP production [61, 74–76]. The indolamine also increases the activity of the respiratory complexes, counteracts the oxidative stress, and maintains the mitochondrial GSH pool under different experimental conditions both in vivo and in vitro . These antioxidant and anti-inflammatory properties of melatonin are relevant in mitochondrial physiology, and they may play a neuroprotective role in PD .
Concerning dendritic spine loss, Roberts and DiFiglia , Ingham et al. , Pickel et al. , Stephens et al. , and Avila-Costa et al. [33, 70] found that the proportion of axospinous synapses was significantly reduced in the ipsilateral striatum of the 6-OHDA lesioned rats, type of synapse that also decrease in caudate nucleus of PD patients [80–82]. Here we found an evident loss of dendritic spines in 6OHDA-lesioned and LD-treated rats. Schuster et al.  reported that, after 6-OHDA lesion and LD treatment, the rats presented a marked loss of dendritic spines in the lesioned striatum, suggesting that the prevention of dendritic spine loss is crucial to impede AIMs. However, it is possible that the role for spine loss after DA depletion could be adaptive and might prevent degeneration of striatal neurons from a sustained and excessive glutamatergic input in the absence of endogenous DAergic control of the excitatory transmission . There is no doubt that the spine loss response is adaptive because preventing the elevation in dendritic excitability significantly reduces spine loss [84, 85]. Nevertheless, melatonin treatment, here, prevented the dendritic spine loss, a fact that is related to the greater number of TH-positive cells and the functional recovery, making melatonin a plausible candidate to avoid disease progression.
On the other hand, previous reports stand out that nigrostriatal synaptic terminals most commonly form contacts with dendritic spines and less commonly with the somata or dendrites of striatal neurons [86–91]. Following 6-OHDA lesioning, the number of distal dendrite and spine contacts decrease, and consequently there are a greater proportion of more proximal dendrite and soma contacts [91, 92]. More recently, Reynolds et al.  described how stimulation of the SNc induced potentiation of the glutamatergic synapses between the cortex and the striatum that was dependent on activation of DA receptors. The corticostriatal glutamatergic fibres synapse onto the heads of dendritic spines of the striatal neurons, whereas the SNc terminals normally synapse onto the spine necks. As more proximal synapses are believed to elicit greater physiological changes in the target neurons than distal synapses , the more proximal site of termination of the reinnervated DA terminals could enhance the efficiency of DA intensification of glutamatergic transmission. Indeed, Picconi  described that plasticity at the cortical projection onto spiny neurons was altered by selective DA receptor blockade and following DA denervation but restored by LD therapy, at least at the start of treatment [94–96]. Other authors have noted that, following neuroleptic treatment, there is persistent modification in dendrites and spines, especially in the ventral striatum. As lesioning and LD therapy both produce damage , it is possible that this alteration provides the drive for the synaptic remodeling described here and elsewhere [98–100], such as the increment in the number of perforated synapses . We speculate that the altered morphology and function of these terminals not only reflect mechanisms that may compensate for the loss of nigral neurons but may also be important in understanding the molecular processes underlying the dyskinesia induced by LD treatment.
Finally, the incremented number of perforated synapses in the three experimental groups is in agreement with other authors who reported an important proliferation of perforated synapses following harming circumstances, hippocampal kindling , unilateral lesion of the entorhinal cortex , and other brain lesions . These authors suggest that the perforations may function to increase the perimeter surface of the postsynaptic density and also the efficiency of neurotransmission. Furthermore, this kind of synapses are intermediate structures in an ongoing cycle of the breakdown and replacement of synapses that are lost because of a specific lesion, transmission changes, or ageing process . In this way, See et al.  demonstrate that after chronic neuroleptic treatment, which increases the DAergic binding sites, there was a significant increment in the number of perforated synapses. In that report, the authors found an important proliferation in perforated synapses after the lesion, maybe as a consequence of the postsynaptic supersensitivity of the DAergic receptors reported by Ungerstedt , which in turn induces synaptic plasticity, increasing the length of the postsynaptic density . Therefore, selective synaptic changes in shape and function are possibly signs of excitotoxic injury, as observed in diverse neurological diseases and neurodegenerative disorders. Elucidating mechanisms that mediate the synaptic alterations under pathological conditions may be of fundamental importance to understanding mechanisms of neuronal injury. In spite of many new findings, there are still various questions to be answered and further experiments to be done. The mechanisms of synaptic plasticity are still not completely clear: the role of retrograde messengers, details in the molecular cascades leading to gene expression and new protein synthesis or to growth of new synapses, finding the more accurate causal connection between plasticity and various forms of learning, memory, and cell. The use of regulated and anatomically restricted genetic modification, combined with morphologic analysis, should provide a powerful set of tools for elucidating synaptic plasticity mechanisms damage .
In conclusion, the data described in the present study provides further evidence that melatonin acts as a DA regulator impeding partially the DAergic cell death by means of preservation of the striatal neuropile and the dendritic spines, promoting functional recovery, since melatonin-treated rats displayed better motor performance and no dyskinetic alterations, compared to LD-treated rats.
Melatonin displays an important antioxidant property based on its ability to function as a free radical scavenge. Particularly, in contrast to conventional antioxidants, melatonin can rapidly cross the blood-brain barrier after systemic administration. This unique character enables melatonin to directly reach the neuronal compartment . At this point, we have to focus on early, long-term, preventive administration of melatonin. Based on previous and current results, melatonin is more likely the complementary and alternative therapy.
It is important to stand out that levels of melatonin tend to decrease with age in contrast to the increased incidence of neurodegenerative diseases. Aging and neurodegenerative diseases have been proposed as a consequence of the imbalance (physiological or toxin-induced) between oxidant production by the organism and its antioxidant defense system. Other constituents of this antioxidant system have not been found to decrease with age, melatonin being the only one matching this age-related pattern. This points to an increase in free radicals which are endogenously or exogenously produced. The protective effect of melatonin demonstrated in abundant cell culture experiments, together with the in vivo protection against 6-OHDA and MPTP-induced cell damage, makes melatonin a plausible candidate in the prevention of the appearance of these diseases and gives a clue to its use as a treatment to avoid disease progression . Consequently, melatonin administration combines antioxidant capacity along with a tissue-specific TH-inducing effect, which could be beneficial for treating PD.
The authors acknowledge Jesus Espinosa Villanueva and Patricia Aley Medina for their excellent photographical and technical assistance. This paper was supported by PAPCA-Iztacala UNAM no. 30-2010-2011, PAPIIT-DGAPA IN220111, and CONACyT-SNI–118320. Authors also thank the Posgrado en Ciencias Biológicas of Universidad Nacional Autónoma de México.
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