Table of Contents
ISRN Agronomy
Volume 2012, Article ID 367851, 8 pages
Research Article

Callus Induction, Proliferation, and Plantlets Regeneration of Two Bread Wheat (Triticum aestivum L.) Genotypes under Saline and Heat Stress Conditions

1Genetic, Biochemistry & Plant Biotechnology Laboratory, Constantine University, Constantine 25000, Algeria
2Plant Protection & Improvement Laboratory, Centre of Biotechnology of Sfax, University of Sfax, 3018 Sfax, Tunisia
3Biology Department, Faculty of Sciences, Sétif University, Sétif 19000, Algeria

Received 22 August 2011; Accepted 21 September 2011

Academic Editors: W. J. Rogers and Z. Yanqun

Copyright © 2012 Laid Benderradji et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Response of two genotypes of bread wheat (Triticum aestivum), Mahon-Demias (MD) and Hidhab (HD1220), to mature embryo culture, callus production, and in vitro salt and heat tolerance was evaluated. For assessment of genotypes to salt and heat tolerance, growing morphogenic calli were exposed to different concentrations of NaCl (0, 5, 10, and 15 g·L−1) and under different thermal stress intensities (25, 30, 35, and 40°C). Comparison of the two genotypes was reported for callus induction efficiency from mature embryo. While, for salt and heat tolerance, the proliferation efficiency, embryonic efficiency, and regeneration efficiency were used. The results show significant medium and genotype effects for the embryogenesis capacity of calluses induction and plantlets regeneration under saline and thermal stresses. Mahon-Demias showed good callus induction and ability to proliferate and regenerate seedling under heat and salt stress conditions compared to Hidhab. No sizeable differences were observed between the two genotypes at higher salt stress rates. This study will serve as a base line for in vitro screening of several elite wheat cultivars for their ability to induce callus and regenerate plants from mature embryos, and to start selection for tolerance to salinity.