Callus Induction, Proliferation, and Plantlets Regeneration of Two Bread Wheat (Triticum aestivum L.) Genotypes under Saline and Heat Stress Conditions

Table 3

Genotypes mean values of the measured variables at different heat treatments.

Genotypic callus induction efficiency
Genotype	MD				HD
No. incubated embryos	96.0				96.0
No. embryos showing calli	45.0				35.0
Callus efficiency (%)	46.8				36.4
Effect of heat stress (°C)
Genotype	MD				HD
Heat (°C)	25	30	35	40	25	30	35	40
No. of incubated embryos	24	24	24	24	24	24	24	24
No. proliferating calli	15	12	10	08	12	10	08	05
Proliferation efficiency (%)	62.5	50.0	41.6	33.3	50.0	41.6	33.3	20.8
No. calli differentiating shoot	2.0	2.0	1.0	0.0	5.0	3.0	1.0	0.0
Embryogenic efficiency (%)	13.3	16.6	10.0	0.0	41.6	30.0	12.0	0.0
No. calli differentiating root	1.0	0.0	0.0	0.0	1.0	0.0	0.0	0.0
No. regenerating calli	1.0	1.0	0.0	0.0	1.0	0.0	0.0	0.0
Regeneration efficiency (%)	4.1	4.1	0.0	0.0	4.1	0.0	0.0	0.0
No. leaves/plantlet	4.0	3.0	0.0	0.0	2.0	0.0	0.0	0.0
No. roots/plantlet	2.0	2.0	0.0	0.0	2.0	0.0	0.0	0.0
Root length (mm)	25.0	20.0	0.0	0.0	20.0	0.0	0.0	0.0