Efficient Nonviral Gene Therapy Using Folate-Targeted Chitosan-DNA Nanoparticles In Vitro
Figure 4
Cells viability treated with chitosan nanoparticules (a) containing the VR1412 β-gal plasmid DNA. Group 1: negative control (KB cells); Group 2: naked DNA; Group 3: Ch-DNA nanoparticule with a Mw = 5 KDa chitosan; Group 4: Ch-PEG-FA-DNA nanoparticule with a Mw = 5 KDa chitosan; Group 5: ch-DNA nanoparticule with a Mw = 25 KDa chitosan; Group 6: Ch-PEG-FA-DNA with a Mw = 25 KDa chitosan; Group 7: Ch-DNA with a Mw = 50 KDa chitosan; Group 8: Ch-PEG-FA-DNA nanoparticule with a Mw = 50 KDa chitosan; Group 9: Lipofectamine. (b) Chitosan nonconjugated with DNA, Group 1: negative control; Group 3: Mw = 5 KDa chitosan; Group 4: Mw = 5 KDa chitosan combined with FA; Group 5: Mw = 25 KDa Chitosan; Group 6: Mw = 25 KDa chitosan combined with FA; Group 7: Mw = 50 KDa chitosan; Group 8: Mw = 50 KDa chitosan combined with FA; Group 9: lipofectamine. *Statistical significant differences compared with positive control .
