Table of Contents
ISRN Allergy
Volume 2012 (2012), Article ID 375735, 8 pages
http://dx.doi.org/10.5402/2012/375735
Research Article

Epicutaneous Immunotherapy Compared with Sublingual Immunotherapy in Mice Sensitized to Pollen (Phleum pratense)

1DBV Technologies, R&D Department, 92220 Bagneux, France
2HΓ΄pital Necker, Gastroenterology Department, 75014 Paris, France

Received 19 December 2011; Accepted 4 January 2012

Academic Editors: B. M. Stadler and B. Vonakis

Copyright © 2012 Lucie Mondoulet et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. The aim of this study was to compare the efficacy of epicutaneous immunotherapy (EPIT) to sublingual immunotherapy (SLIT) in a model of mice sensitized to Phleum pratense pollen. Methods. BALB/c mice were sensitized by sub-cutaneous route to pollen protein extract mixed treated for 8 weeks, using sham, EPIT, or SLIT. Measurements involved the serological response and cytokine profile from reactivated splenocytes, plethysmography after aerosol challenge to pollen, cell, and cytokine contents in the bronchoalveolar lavages (BALs). Results. After immunotherapy, sIgE was significantly decreased in the treated groups compared to sham ( 𝑃 < 0 . 0 0 1 ), whereas sIgG2a increased with EPIT and SLIT ( 𝑃 < 0 . 0 0 1 and 𝑃 < 0 . 0 0 5 versus sham). Reactivated splenocytes secreted higher levels of Th2 cytokines with sham ( 𝑃 < 0 . 0 1 ). Penh values were higher in sham than EPIT and SLIT. Eosinophil recruitment in BAL was significantly reduced only by EPIT ( 𝑃 < 0 . 0 1 ). Conclusion. In this model of mice sensitized to pollen, EPIT was at least as efficient as SLIT.