Review Article

The Stemness Phenotype Model

Table 2

Detection of CSCs in established lung cancer cell lines.

Cell linePercentageMethodReference

LHK2, 1-87, A549, Lc8170.4% to 2.8%SP[23]
A5490.98%CD133[24]
H4461%CD133[24]
A549>45%Cloning and tumorigenic analyses[25]
H446>45%Cloning and tumorigenic analyses[25]
A54924%SP[26]
H460, H23, HTB-58, A549, H441, and H21701.5% to 6.1%SP[27]
NCI-H82, H146, H526, A549, and H4600.8% to 1%SP[28]
H446 6 . 3 ± 0 . 1 SP[29]
NSCLC cell lines H460, H125, H322, H358average of 2% ( 2 . 1 6 ± 1 . 2 8 )Aldefluor followed by clonogenic assays[30]
A549, H1299, CCL-1, CCL-5, C2990.3% to 1%CD133+ follow serum free culture[31]
60 primary tissue samples0.02% to a maximum of 35%CD133+ESA+[32]