Table of Contents
ISRN Gastroenterology
Volume 2012, Article ID 394545, 6 pages
http://dx.doi.org/10.5402/2012/394545
Research Article

Lowly Expressed Ribosomal Protein S19 in the Feces of Patients with Colorectal Cancer

1Department of Anesthesiology, Sijhih Cathay General Hospital, New Taipei 22174, Taiwan
2School of Medicine, Fu Jen Catholic University, New Taipei 24257, Taiwan
3Department of Internal Medicine, Cathay General Hospital, Taipei 10630, Taiwan
4School of Medicine, Taipei Medical University, Taipei 11031, Taiwan
5Department of Medical Research, Cathay General Hospital, Taipei 10630, Taiwan
6Department of Biochemistry, National Defense Medical Center, Taipei 11490, Taiwan
7Department of Surgery, Taipei-Veterans General Hospital, Taipei 11217, Taiwan
8School of Medicine, National Yang Ming University, Taipei 11221, Taiwan
9Department of Internal Medicine, Hsinchu Cathay General Hospital, Hsinchu 30060, Taiwan

Received 16 August 2011; Accepted 4 October 2011

Academic Editors: A. Amedei and S. Grösch

Copyright © 2012 Chih-Cheng Chien et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Colorectal cancer (CRC) has become one of the most common fatal cancers. CRC tumorigenesis is a complex process involving multiple genetic changes to several sequential mutations or molecular alterations. P53 is one of the most significant genes; its mutations account for more than half of all CRC. Therefore, understanding the cellular genes that are directly or indirectly related to p53 is particularly crucial for investigating CRC tumorigenesis. In this study, a p53-related ribosomal protein, ribosomal protein S19 (RPS19), obtained from the feces of CRC patients is evaluated by using specifically quantitative real-time PCR and knocked down in the colonic cell line by gene silencing. This study found that CRC patients with higher expressions of RPS19 in their feces had a better prognosis and consistent expressions of RPS19 and BAX in their colonic cells. In conclusion, the potential mechanism of RPS19 in CRC possibly involves cellular apoptosis through the BAX/p53 pathway, and the levels of fecal RPS19 may function as a prognostic predictor for CRC patients.