Table of Contents
ISRN Zoology
Volume 2012, Article ID 498731, 8 pages
Research Article

Sequence Analysis of Mitochondrial DNAs of 12S rRNA, 16S rRNA, and Cytochrome Oxidase Subunit 1(COI) Regions in Slow Lorises (Genus Nycticebus) May Contribute to Improved Identification of Confiscated Specimens

1Asian Wildlife Research Center Foundation, Yokohama 231-0057, Japan
2Department of Molecular Cell Biology, Research Center for Medical Sciences, Institute of DNA Medicine, Jikei University School of Medicine, Tokyo 105-8461, Japan
3Department of Pathology, Jikei University School of Medicine, Tokyo 105-8461, Japan
4Nasu World Monkey Park, Tochigi 325-0001, Japan
5Core Research Facilities, Research Center for Medical Sciences, Jikei University School of Medicine, Tokyo 105-8461, Japan

Received 21 October 2011; Accepted 1 December 2011

Academic Editors: J. J. Gros-Louis, S. P. Henzi, V. Ketmaier, and H. H. Segers

Copyright © 2012 Hiroko Somura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The slow loris (Nycticebus) is a prosimian that is popular among exotic pet lovers. In Japan, many slow lorises have been imported illegally. Prosimians that have been confiscated in raids are protected in Japanese zoos, and the number of such animals has increased. In most cases, the country of origin remains unknown and even the species can be difficult to identify from the animal’s physical appearance alone. We have attempted to resolve this problem by using DNA analysis. DNA samples of five species, consisting of the Pygmy slow loris (Nycticebus pygmaeus), Bengal slow loris (Nycticebus bengalensis), Sunda slow loris (Nycticebus coucang), Javan slow loris (Nycticebus javanicus), and Bornean slow loris (Nycticebus menagensis), were extracted, amplified, and the nucleotide sequences of mitochondrial 12S rRNA, 16S rRNA, and the cytochrome oxidase subunit 1(COI) regions were compared. Differences of nucleic acid sequences of representative individuals were demonstrated.