An Approach to Visualize the Deformation of the Intermediate Filament Cytoskeleton in Response to Locally Applied Forces

<table>Schematic of the experimental setup. (a) A cell transiently expressing GFP-Vimentin is located. (b) A grid (3.2 <i>μ</i>m spacing with 0.5 <i>μ</i>m lines) is photobleached into the IF CSK. (c) The nodes (yellow dots) of the grid are tracked over time. (d) Side view of the combined AFM-LSCM. An AFM cantilever is used to apply a constant downward mechanical force to the centre of the nucleus in a cell expressing GFP-vimentin. Simultaneous LSCM is employed to image the deformation of the IF CSK on an inverted microscope.</table>

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Figure 1

Figure 1: An Approach to Visualize the Deformation of the Intermediate Filament Cytoskeleton in Response to Locally Applied Forces 