Table of Contents
ISRN Microbiology
Volume 2012 (2012), Article ID 517524, 8 pages
http://dx.doi.org/10.5402/2012/517524
Research Article

Thermostable and Alkalistable Xylanases Produced by the Thermophilic Bacterium Anoxybacillus flavithermus TWXYL3

Department of Biological Sciences, Idaho State University, P.O. Box 8007, Pocatello, ID 83209, USA

Received 26 June 2012; Accepted 19 July 2012

Academic Editors: B. Ayo and J. Ruiz-Herrera

Copyright © 2012 Joshua T. Ellis and Timothy S. Magnuson. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. Z. X. Lu, A. Laroche, and H. C. Huang, “Segregation patterns for integration and expression of Coniothyrium minitans xylanase gene in Arabidopsis thaliana transformants,” Botanical Bulletin of Academia Sinica, vol. 45, no. 1, pp. 23–31, 2004. View at Google Scholar · View at Scopus
  2. G. Mamo, M. Thunnissen, R. Hatti-Kaul, and B. Mattiasson, “An alkaline active xylanase: insights into mechanisms of high pH catalytic adaptation,” Biochimie, vol. 91, no. 9, pp. 1187–1196, 2009. View at Publisher · View at Google Scholar · View at Scopus
  3. P. Dwivedi, V. Vivekanand, N. Pareek, A. Sharma, and R. P. Singh, “Bleach enhancement of mixed wood pulp by xylanase-laccase concoction derived through co-culture strategy,” Applied Biochemistry and Biotechnology, vol. 160, no. 1, pp. 255–268, 2010. View at Publisher · View at Google Scholar · View at Scopus
  4. T. S. Magnuson, Biochemical and genetic studies on the lignocellulose degradation system of streptomyces viridosporus T7A [dissertation thesis], University of Idaho, Moscow, Russia, 1996.
  5. C. Valls and M. B. Roncero, “Using both xylanase and laccase enzymes for pulp bleaching,” Bioresource Technology, vol. 100, no. 6, pp. 2032–2039, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. S. Nagar, V. K. Gupta, D. Kumar, L. Kumar, and R. C. Kuhad, “Production and optimization of cellulase-free, alkali-stable xylanase by Bacillus pumilus SV-85S in submerged fermentation,” Journal of Industrial Microbiology and Biotechnology, vol. 37, no. 1, pp. 71–83, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. M. Q. Wang, C. Saricks, and M. Wu, “Fuel-Cycle Fossil Energy Use and Greenhouse Gas Emissions of Fuel Ethanol Produced from U.S. Midwest Corn,” Prepared for Illinois Department of Commerce and Community Affairs, Center for Transportation Research, Argonne National Laboratory, Argonne, Ill, USA, 1997.
  8. S. Ahmed, S. Riaz, and A. Jamil, “Molecular cloning of fungal xylanases: an overview,” Applied Microbiology and Biotechnology, vol. 84, no. 1, pp. 19–35, 2009. View at Publisher · View at Google Scholar · View at Scopus
  9. I. D. Choi, H. Y. Kim, and Y. J. Choi, “Gene cloning and characterization of α-glucuronidase of Bacillus stearothermophilus No. 236,” Bioscience, Biotechnology and Biochemistry, vol. 64, no. 12, pp. 2530–2537, 2000. View at Google Scholar · View at Scopus
  10. G. M. Zhang, J. Huang, G. R. Huang, L. X. Ma, and X. E. Zhang, “Molecular cloning and heterologous expression of a new xylanase gene from Plectosphaerella cucumerina,” Applied Microbiology and Biotechnology, vol. 74, no. 2, pp. 339–346, 2007. View at Publisher · View at Google Scholar · View at Scopus
  11. H. Wei, Q. Xu, L. E. Taylor, J. O. Baker, M. P. Tucker, and S. Y. Ding, “Natural paradigms of plant cell wall degradation,” Current Opinion in Biotechnology, vol. 20, no. 3, pp. 330–338, 2009. View at Publisher · View at Google Scholar · View at Scopus
  12. S. Marques, L. Alves, S. Ribeiro, F. M. Gírio, and M. T. Amaral-Collaço, “Characterization of a thermotolerant and alkalotolerant xylanase from a Bacillus sp.,” Applied Biochemistry and Biotechnology A, vol. 73, no. 2-3, pp. 159–172, 1998. View at Google Scholar · View at Scopus
  13. C. Mawadza, R. Hatti-Kaul, R. Zvauya, and B. Mattiasson, “Purification and characterization of cellulases produced by two Bacillus strains,” Journal of Biotechnology, vol. 83, no. 3, pp. 177–187, 2000. View at Publisher · View at Google Scholar · View at Scopus
  14. A. S. S. Ibrahim and A. I. El-diwany, “Isolation and identification of new cellulases producing thermophilic bacteria from an Egyptian hot spring and some properties of the crude enzyme,” Australian Journal of Basic and Applied Sciences, vol. 1, pp. 473–478, 2007. View at Google Scholar
  15. M. Kambourova, R. Mandeva, I. Fiume, L. Maurelli, M. Rossi, and A. Morana, “Hydrolysis of xylan at high temperature by co-action of the xylanase from Anoxybacillus flavithermus BC and the β-xylosidase/α-arabinosidase from Sulfolobus solfataricus Oα,” Journal of Applied Microbiology, vol. 102, no. 6, pp. 1586–1593, 2007. View at Publisher · View at Google Scholar · View at Scopus
  16. A. Pakpitcharoen, K. Potivejkul, P. Kanjanavas, S. Areekit, and K. Chansiri, “Biodiversity of thermotolerant Bacillus sp. producing biosurfactants, biocatalysts, and antimicrobial agents,” ScienceAsia, vol. 34, no. 4, pp. 424–431, 2008. View at Publisher · View at Google Scholar · View at Scopus
  17. P. Biely, D. Mislovičová, and R. Toman, “Soluble chromogenic substrates for the assay of endo-1,4-β-xylanases and endo-1,4-β-glucanases,” Analytical Biochemistry, vol. 144, no. 1, pp. 142–146, 1985. View at Google Scholar · View at Scopus
  18. M. C. T. Duarte, A. C. A. Pellegrino, E. P. Portugal, A. N. Ponezi, and T. T. Franco, “Characterization of alkaline xylanases from Bacillus pumilus,” Brazilian Journal of Microbiology, vol. 31, no. 2, pp. 90–94, 2000. View at Google Scholar · View at Scopus
  19. B. H. Howard, G. Jones, and M. R. Purdom, “The pentosanases of some rumen bacteria,” The Biochemical Journal, vol. 74, pp. 173–180, 1960. View at Google Scholar · View at Scopus
  20. C. C. Lee, R. E. Kibblewhite-Accinelli, M. R. Smith, K. Wagschal, W. J. Orts, and D. W. S. Wong, “Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme,” Current Microbiology, vol. 57, no. 4, pp. 301–305, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. A. Sharma, S. Adhikari, and T. Satyanarayana, “Alkali-thermostable and cellulase-free xylanase production by an extreme thermophile Geobacillus thermoleovorans,” World Journal of Microbiology and Biotechnology, vol. 23, no. 4, pp. 483–490, 2007. View at Publisher · View at Google Scholar · View at Scopus
  22. P. K. Smith, R. I. Krohn, and G. T. Hermanson, “Measurement of protein using bicinchoninic acid,” Analytical Biochemistry, vol. 150, no. 1, pp. 76–85, 1985. View at Google Scholar · View at Scopus
  23. L. Weinstein and P. Albersheim, “Structure of plant-cell walls .IX. Purification and partial characterization of a wall-degrading endoarabanase and an arabinosidase from Bacillus subtilis,” Plant Physiology, vol. 63, pp. 425–432, 1979. View at Google Scholar
  24. R. P. Anex, L. R. Lynd, M. S. Laser, A. H. Heggenstaller, and M. Liebman, “Potential for enhanced nutrient cycling through coupling of agricultural and bioenergy systems,” Crop Science, vol. 47, no. 4, pp. 1327–1335, 2007. View at Publisher · View at Google Scholar · View at Scopus
  25. S. K. Lee, H. Chou, T. S. Ham, T. S. Lee, and J. D. Keasling, “Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels,” Current Opinion in Biotechnology, vol. 19, no. 6, pp. 556–563, 2008. View at Publisher · View at Google Scholar · View at Scopus
  26. M. B. Sticklen, “Expediting the biofuels agenda via genetic manipulations of cellulosic bioenergy crops,” Biofuels, Bioproducts and Biorefining, vol. 3, no. 4, pp. 448–455, 2009. View at Publisher · View at Google Scholar · View at Scopus
  27. Y. Liang, Z. Feng, J. Yesuf, and J. W. Blackburn, “Optimization of growth medium and enzyme assay conditions for crude cellulases produced by a novel thermophilic and cellulolytic bacterium, Anoxybacillus sp. 527,” Applied Biochemistry and Biotechnology, vol. 160, no. 6, pp. 1841–1852, 2010. View at Publisher · View at Google Scholar · View at Scopus
  28. D. Dodd and I. K. O. Cann, “Enzymatic deconstruction of xylan for biofuel production,” GCB Bioenergy, vol. 1, pp. 2–17, 2009. View at Google Scholar
  29. P. C. Badger, Ethanol from Cellulose: A General Review Reprinted from: Trends in New Crops and New Uses, Edited by J. Janick and A. Whipkey, ASHS Press, Alexandria, VA, USA, 2002.
  30. D. J. Lane, 16S/23S rRna Sequencing. Nucleic Acid Techniques. Bacterial Systematics, Edited by Stackebrandt E., Goodfellow M., John Wiley and Sons, New York, NY, USA, 1991.
  31. I. Kimura, H. Sasahara, and S. Tajima, “Purification and characterization of two xylanases and an arabinofuranosidase from Aspergillus sojae,” Journal of Fermentation and Bioengineering, vol. 80, no. 4, pp. 334–339, 1995. View at Publisher · View at Google Scholar · View at Scopus
  32. H. Schagger and G. Von Jagow, “Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa,” Analytical Biochemistry, vol. 166, no. 2, pp. 368–379, 1987. View at Google Scholar · View at Scopus
  33. S. Dulger, Z. Demirbag, and A. O. Belduz, “Anoxybacillus ayderensis sp. nov. and Anoxybacillus kestanbolensis sp. nov,” International Journal of Systematic and Evolutionary Microbiology, vol. 54, no. 5, pp. 1499–1503, 2004. View at Publisher · View at Google Scholar · View at Scopus
  34. H. Matsui, K. Ogata, K. Tajima et al., “Phenotypic characterization of polysaccharidases produced by four Prevotella type strains,” Current Microbiology, vol. 41, no. 1, pp. 45–49, 2000. View at Google Scholar · View at Scopus
  35. P. L. Dimitrov, M. S. Kambourova, R. D. Mandeva, and E. I. Emanuilova, “Isolation and characterization of xylan-degrading alkali-tolerant thermophiles,” FEMS Microbiology Letters, vol. 157, no. 1, pp. 27–30, 1997. View at Publisher · View at Google Scholar · View at Scopus
  36. T. Collins, C. Gerday, and G. Feller, “Xylanases, xylanase families and extremophilic xylanases,” FEMS Microbiology Reviews, vol. 29, no. 1, pp. 3–23, 2005. View at Publisher · View at Google Scholar · View at Scopus
  37. Y. Liang, J. Yesuf, and Z. Feng, “Toward plant cell wall degradation under thermophilic condition: a unique microbial community developed originally from swine waste,” Applied Biochemistry and Biotechnology, vol. 161, no. 1–8, pp. 147–156, 2010. View at Google Scholar · View at Scopus
  38. A. L. Demain, M. Newcomb, and J. H. D. Wu, “Cellulase, clostridia, and ethanol,” Microbiology and Molecular Biology Reviews, vol. 69, no. 1, pp. 124–154, 2005. View at Publisher · View at Google Scholar · View at Scopus