Figure 5: Nck, N-WASP and ARP3 are localized in newly formed circular ruffles upon PDGF stimulation in mouse C3H10T1/2 fibroblasts. Nck (green) and N-WASP (red) are localized in the cytoplasmic pool and in the nucleus of serum-starved cells ((a), (b), (c)). After 15 minutes of stimulation with PDGF-BB, circular ruffles are formed and Nck (green) and N-WASP (red) co-localize in circular ruffles ((d), (e), (f)). Also ARP3 (g) is localized in newly formed F-actin structures (h) such as circular ruffles and lamella (arrows). Bar represents 10 μm.