Implementation of Quality by Design for the Development and Validation of Pioglitazone Hydrochloride by RP-UPLC with Application to Formulated Forms

Figure 5

Chromatograms obtained for method development using Acquity BEH C18, (100 × 2.1) mm, 1.7 μm column at different pH conditions.

(a) ACN : Buffer (pH 3.2) (20 : 80% v/v)

(b) ACN : Buffer (pH 4.2) (20 : 80% v/v)

(c) ACN : Buffer (pH 2.2) (20 : 80% v/v)

(d) ACN : Buffer (pH 5.5) (20 : 80% v/v)