Research Article
Implementation of Quality by Design for the Development and Validation of Pioglitazone Hydrochloride by RP-UPLC with Application to Formulated Forms
Table 2
Observation and remarks of method development for Acquity BEH C18, (100 × 2.1) mm, 1.7 μm column.
| S. No. | Trails taken | Observations | Remarks |
| 1 | ACN : buffer (pH 3.2) (20 : 80% v/v) | Symmetrical peak | Satisfactory | 2 | ACN : water (20 : 80% v/v) | Asymmetrical peak with tailing and early elution | Not Satisfactory | 3 | Methanol : water (20 : 80% v/v) | Very broad peak | Not Satisfactory | 4 | Methanol : buffer (pH 3.2) (20 : 80% v/v) | Broad peak | Not Satisfactory |
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