Research Article

Implementation of Quality by Design for the Development and Validation of Pioglitazone Hydrochloride by RP-UPLC with Application to Formulated Forms

Table 2

Observation and remarks of method development for Acquity BEH C18, (100 × 2.1) mm, 1.7 μm column.

S. No.Trails taken ObservationsRemarks

1ACN : buffer (pH 3.2) (20 : 80% v/v)Symmetrical peakSatisfactory
2ACN : water (20 : 80% v/v)Asymmetrical peak with tailing and early elutionNot Satisfactory
3Methanol : water (20 : 80% v/v)Very broad peakNot Satisfactory
4Methanol : buffer (pH 3.2) (20 : 80% v/v)Broad peakNot Satisfactory