Implementation of Quality by Design for the Development and Validation of Pioglitazone Hydrochloride by RP-UPLC with Application to Formulated Forms

Table 6

Results of precision study.

Intra-day precision (𝑛=7)
Concentration injected; (𝜇g mL^−1)	Mean area ± SD	RSD^a	Mean RT ± SD	RSD^b
100	222879 ± 1648	0.74	2.19 ± 0.004	0.16
200	502609 ± 590	0.12	2.19 ± 0.007	0.32
300	755267 ± 2276	0.30	2.20 ± 0.002	0.09
Inter-day precision (𝑛=5)
Concentration injected (𝜇g mL^−1)	Mean area ± SD	RSD^a	Mean RT ± SD	RSD^b
100	223992 ± 1130	0.50	2.19 ± 0.024	1.08
200	501345 ± 3070	0.61	2.19 ± 0.015	0.67
300	754190 ± 1555	0.21	2.18 ± 0.011	0.52

^aRelative standard deviation based on peak area.
^bRelative standard deviation based on retention time.