The Global Transcriptional Response of Isolated Human Islets of Langerhans to Glucagon-Like Peptide-1 Receptor Agonist Liraglutide
Figure 1
Viability and function of isolated human islets. Human islets were cultured in medium containing GLP-1 (10 nM), or vehicle for 22 h. (a) Cell viability was determined using the Living/Dead Viability/Cytotoxicity Kit. Living cells were identified by a green staining (Calcein AM staining), while dead cells were identified by a brown nuclear staining (Ethidium homodimer-1 staining). (b) The insulin accumulation into the culture medium was determined by RIA, and normalized to the total protein content in the cell pellet. Statistical significance of the data was evaluated by Student’s t-test. *= .
