The Escherichia coli GcvB sRNA Uses Genetic Redundancy to Control cycA Expression
Figure 3
(a) Effects of gcvB mutant alleles on cycA-lacZ expression. WT and ΔgcvB λcycA-lacZ lysogens transformed with the indicated gcvB alleles were grown in LB (+Amp for transformants) to mid-log phase and assayed for β-galactosidase. (b) Northern analysis of GcvB. RNA was isolated from WT, ΔgcvB, or ΔgcvB transformed with the indicated gcvB alleles and probed with either a DIG-labeled GcvB or 5S rRNA specific DNA probe. Numbers above each lane indicate levels of GcvB relative to WT.
