Research Article

The Escherichia coli GcvB sRNA Uses Genetic Redundancy to Control cycA Expression

Figure 6

GcvB represses cycA mRNA by an antisense mechanism. WT and ΔgcvB λcycA-lacZ, 𝜆 𝑐 𝑦 𝑐 𝐴 2 9 G -lacZ, 𝜆 𝑐 𝑦 𝑐 𝐴 3 0 T -lacZ, and 𝜆 𝑐 𝑦 𝑐 𝐴 2 4 G G -lacZ lysogens transformed with the indicated plasmids were grown in LB (+Amp for transformants) to mid-log phase and assayed for β-galactosidase.
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