Assessment of Pseudoaffinity Chromatography Using Textile Dyes for Isolation of Buffalo Pituitary Luteinizing Hormone

Table 1

Quantitative elution pattern of protein on different dye-agarose columns.

S. no.	Name of the dye	Changing the composition of the buffers (%)	Changing the pH of the buffers (%)	Changing the ionic strength of the buffers (%)
(1)	Cibacron blue 3GA	70.46	79.03	84.94
(2)	Reactive blue	53	61.13	80.52
(3)	Reactive brown	47.52	55	87.64
(4)	Reactive yellow	95	96.1	96.76
(5)	Reactive red	—	39.76	49.82/92.7 (arginine)
(6)	Reactive green	40.48	74	77.94